目的 探讨恒温干燥箱在砷铈催化分光光度法检测尿碘中代替恒温消解仪对尿样消化处理的可行性。方法 使用恒温干燥箱代替恒温消解仪消解尿样品,所用试剂和其他操作步骤同标准方法,分析方法检出限、标准曲线的线性范围、精密度、加标回收、标准物质测定结果,并比较两者处理30份尿样检测结果。结果 使用恒温干燥箱处理尿样后,其尿碘浓度范围在 0～300 μg/L,相关系数∣r∣=0.9995～1.0000,方法检出最低值1.9 μg/L(取样量为0.25 ml);尿碘浓度范围在 300～1 200 μg/L,相关系数∣r∣=0.9992～0.9999,加标回收率为97.3%～100%,精密度(RSD)为1.09%～1.43%。使用本消解方法与标准方法同时测定30份不同浓度的尿样,检测结果差异无统计学意义(t=1.170,P＞0.05)。结论 电热恒温干燥箱进行尿样消解,温度均匀性好,其方法的精密度和准确度均能满足要求。

Objective To explore the feasibility of using constant temperature drying oven instead of constant temperature digester in the determination of urinary iodine by arsenic cerium catalytic spectrophotometry. Methods Using constant temperature drying oven instead of constant temperature digestion apparatus to digest urine samples, the reagents and other operation steps were the same as the standard method.The detection limit, linear range of standard curve, precision, recovery of standard addition and determination results of reference materials were analyzed.The detection results of 30 urine samples were compared between the two methods. Results The range of urine iodine concentration was 0-300μg/L, the correlation coefficient∣r∣=0.9995-1.0000, the lowest detection value was 1.9 μg/L (sampling volume was 0.25 ml).The range of urine iodine concentration was 300-1 200μg/L,the correlation coefficient∣r∣was 0.9992-0.9999, the recovery rate was 97.3% - 100%, and the precision (RSD) was 1.09%-1.43%.There was no significant difference between the two methods (t = 1.170, P> 0.05). Conclusion The temperature uniformity is good, and the precision and accuracy of the method can meet the requirements.