目的 了解滁州市2018~2019年HIV抗体初筛阳性标本的免疫印迹类型分布情况,为临床诊治提供科学依据。方法 收集滁州市2018~2019年200份初筛HIV抗体阳性标本进行免疫印迹(WB)检测。结果 200份阳性标本经免疫印迹确证,其中阳性168份,阳性率84.00%,阴性22份(占11.00%),不确定10份(占5.00%)。不同机构的筛查阳性率差异统计学意义(χ2=44.561,P<0.001),阳性率最低为采供血机构(26.67%)。168份HIV抗体确认阳性标本,58份标本出现8个全带,占34.52%,gp160、gp120、p66和p51的阳性率最高,其中gp160的阳性率为100.00%,p17条带阳性率最低(41.67%)。10份不确定标本,gpl60条带出现率最高, 5例随访成功,其中3例按规定判定为阳性。结论 免疫印迹确证试验能排除筛查实验的假阳性,应高度重视不确定结果,排除艾滋的窗口期和终末期。
Abstract
Objective To investigate the distribution of immunoblotting types of HIV antibody screening positive samples in Chuzhou City from 2018 to 2019,so as to provide scientific basis for clinical diagnosis and treatment. Methods A total of 200 HIV antibody positive samples were collected from Chuzhou City from 2018 to 2019 for Western blot (WB) detection. Results The 200 positive samples were confirmed by WB,among which 168 were positive (84.00%),22 were negative (11.00%),and 10 were uncertain (5.00%).The difference in positive rate of screening in different institutions was significant (χ2=44.561,P<0.001),and the lowest positive rate was in the blood collection and supply institutions (26.67%).Among samples confirmed positive for HIV antibody,there were 58 (34.52%) of the 8 all-band specimens,with the highest positive rate of gp160,gp120,p66 and p51,among which gp160 was 100.00% and that of p17 was the lowest (41.67%).There was no significant difference in band frequency between different age groups.Among the uncertain samples,the occurrence rate of band gp160 was the highest.5 cases were followed up successfully,among which 3 cases were judged as positive according to the regulations. Conclusion Western blot confirmatory test can eliminate false positive of screening test.We should attach great importance to uncertain results and exclude the window period and end-stage of AIDS.
关键词
HIV /
筛查 /
确证检测 /
免疫印迹试验
Key words
HIV /
Screening /
Confirmed inspection /
Western blot test
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