目的 了解蚌埠市水产品中致病性弧菌的污染情况、副溶血弧菌毒力基因携带情况,为食源性疾病防控提供依据。方法 采集蚌埠市农贸市场、超市、饭店水产品样品,依据《2018年国家食品污染和有害因素风险监测工作手册》对水产品中的副溶血弧菌、霍乱弧菌、创伤弧菌、溶藻弧菌、河弧菌进行分离培养鉴定;采用荧光PCR方法检测副溶血弧菌不耐热溶血素(TLH)、耐热性溶血素(TDH)、耐热相关溶血素(TRH)和TOXR基因。结果 共采集水产品2 00份,检出致病性弧菌93株,阳性数为80份,阳性率为40.0%,其中副溶血弧菌检出率22.5%、霍乱弧菌检出率3.5%、创伤弧菌检出率0.5%、溶藻弧菌检出率17.0%、河弧菌检出率3.0%;全部副溶血弧菌毒力基因均为阴性。结论 5种致病性弧菌在蚌埠市水产品中均有不同程度的污染,其中以副溶血弧菌和溶藻弧菌最为严重。

Objective To understand the contamination of pathogenic Vibrio in aquatic products and the carrying of virulence genes of Vibrio parahaemolyticus in Bengbu City,so as to provide basis for the prevention and control of foodborne diseases. Methods The samples of aquatic products from farmers' markets,supermarkets and restaurants in Bengbu City were collected,and Vibrio parahaemolyticus,Vibrio cholerae,Vibrio vulnificus,Vibrio alginolyticus and Vibrio fluvialis in aquatic products were isolated,cultured and identified according to the National Handbook for Risk Monitoring of Food Pollution and Harmful Factors in 2018.The thermolabile hemolysin (TLH),thermostable direct haemolysin(TDH),thermolabile related hemolysin (TRH) and TOXR gene were detected by fluorescent PCR. Results A total of 200 aquatic products were collected,93 strains of pathogenic Vibrio were detected,80 of which were positive,with a positive rate of 40.0%.Among them,the detection rates of Vibrio parahaemolyticus,Vibrio cholerae,Vibrio vulnificus,Vibrio alginolyticus and Vibrio fluvialis were 22.5%,3.5%,0.5%,17.0% and 3.0%,respectively.All virulence genes of Vibrio parahaemolyticus were negative. Conclusion The aquatic products in Bengbu City are polluted by five kinds of pathogenic Vibrio to different degrees,among which Vibrio parahaemolyticus and Vibrio alginolyticus are the most serious.