目的 分析北京市某区导致儿童感染性腹泻的病原谱,为儿童感染性腹泻科学防控提供参考。方法 收集2021年12月至2022年11月北京市某区儿童感染性腹泻病例基础信息和粪便标本,进行腹泻常见12种细菌和3种病毒核酸的荧光PCR检测,构建病原谱,分析病原流行特征。结果 共收集200例儿童感染性腹泻标本,病原总检出率为35.00%(70/200)。其中细菌检出率为26.00%(52/200),检出率前3位分别为致泻大肠埃希菌(12.50%,25/200)、沙门菌(9.00%,18/200)和副溶血性弧菌(5.50%,11/200);病毒检出率为10.50%(21/200),轮状病毒和诺如病毒检出率分别为4.00%(8/200)和6.50%(13/200)。在混合感染中,2种细菌混合感染检出率为5.00%(10/200),1种细菌和1种病毒混合感染检出率为1.50%(3/200)。不同季节细菌病原(χ2=16.225,P=0.001)和病毒检出率(χ2=35.184,P<0.001)差异均有统计学意义。结论 致泻大肠埃希菌、沙门菌、副溶血性弧菌、轮状病毒、诺如病毒在北京市某区儿童感染性腹泻病例中具有较高的流行强度。轮状病毒、沙门菌和副溶血性弧菌与其他细菌混合感染的情况在儿童腹泻中应引起重视。
Abstract
Objective To analyze the pathogenic spectrum of infectious diarrhea in children in a certain district of Beijing City,so as to provide reference for scientific prevention and control of children's infectious diarrhea. Methods Basic information and fecal samples of children with infectious diarrhea in a certain district of Beijing City from December 2021 to November 2022 were collected.The nucleic acids of 12 common bacteria and 3 viruses that caused diarrhea were detected by fluorescent PCR.Pathogenic spectrum was constructed and epidemic characteristics were analyzed. Results A total of 200 specimens of infectious diarrhea in children were collected,with a total pathogen detection rate of 35.00% (70/200).The detection rate of bacteria was 26.00% (52/200),with the top three detection rates being Escherichia coli (12.50%,25/200),Salmonella (9.00%,18/200),and Vibrio parahaemolyticus (5.50%,11/200),respectively.The virus detection rate was 10.50% (21/200),with rotavirus and norovirus detection rates of 4.00% (8/200) and 6.50% (13/200),respectively.In mixed infection,the detection rate of mixed infection of two bacteria was 5.00% (10/200),and the detection rate of mixed infection of one bacteria and one virus was 1.50% (3/200).There was statistically significant differences in the detection rate of bacterial pathogens (χ2=16.225,P=0.001) and viruses (χ2=35.184,P<0.001) in different seasons. Conclusion Diarrhoeal Escherichia coli,Salmonella,Vibrio parahaemolyticus,rotavirus,and norovirus have a high epidemic intensity in children with infectious diarrhea in a district of Beijing City.The coinfection of rotavirus,Salmonella and Vibrio parahaemolyticus with other bacterial pathogens should be given attention in children's diarrhea.
关键词
儿童感染性腹泻 /
病原谱 /
荧光PCR
Key words
Infectious diarrhea in children /
Pathogenic spectrum /
Fluorescent PCR
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