目的 了解2020年合肥地区一起幼儿园疱疹性咽峡炎(HA)聚集性疫情病原体型别及基因进化情况。方法 采用实时荧光定量RT-PCR方法对11份咽拭子标本进行肠道病毒(EV)病原检测,柯萨奇病毒A6型(CVA6)核酸阳性的标本经传统RT-PCR扩增VP1基因,进行序列测定,用MEGA 6.0软件构建基因进化树。结果 11份咽拭子标本中6份CVA6核酸阳性,6条基因序列均为CVA6 D3亚型。结论 CVA6 D3型是引起本次幼儿园HA疫情的病原体,应加强对肠道病毒的监测,重视在幼托机构暴发的此类疫情。
Abstract
Objective To understand the pathogen type and gene evolution of herpangina (HA) cluster in a kindergarten in Hefei City in 2020. Methods Enterovirus (EV) pathogen was detected on 11 throat swabs by real-time fluorescent quantitative RT-PCR.The VP1 area gene of coxsackievirus A6 (CVA6) positive specimens were amplified by conventional RT-PCR,and the sequence was determined.MEGA 6.0 software was used to construct the phylogenetic tree. Results Among 11 throat swab samples,6 were positive for CVA6 nucleic acid.All 6 gene sequences belonged to the CVA6 D3 subtype. Conclusion CVA6 D3 subtype is the pathogen of the HA outbreak in the kindergarten.Enterovirus surveillance should be strengthened and attention should be paid to such outbreaks in child care facilities.
关键词
疱疹性咽峡炎 /
柯萨奇病毒A组6型 /
聚集性疫情 /
基因型
Key words
Herpangina /
Coxsackievirus A6 /
Outbreak in clusters /
Genotype
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基金
2019年应用医学研究项目(合卫科教(2019)172号-yb018 )
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