目的 分析安庆市2022—2023年发热伴血小板减少综合征(SFTS)病原学检测结果,为SFTS的科学防控提供基础资料。方法 采用RT-PCR方法对安庆市医疗卫生机构临床送检的520份疑似SFTS病例血清标本开展大别班达病毒(DBV)核酸检测,采用SPSS 25.0对阳性病例流行病学信息进行分析,对部分分离到的病毒株进行S基因测序并构建系统进化树。结果 502份疑似SFTS病例血清样本中,DBV核酸阳性151份,阳性检出率为30.08%;其中男性阳性检出率(27.83%)与女性阳性检出率(31.72%)比为0.88∶1;各年龄组阳性检出率差异有统计学意义(χ2=15.271,P=0.033),71~80岁组人群占比最高(占38.84%);农民病例占比最高(占84.77％);病例时间分布在4—10月,其中5月份病例占比最高(占27.82%);不同县(区)间的阳性检出率差异有统计学意义(χ2=18.080,P=0.021),其中太湖县病例占比最高(占34.75%)。测序获得21份毒株S片段基因全长序列,其中F型10株,A型5株,E型5株,B型1株。结论 安庆市及周边县(区)SFTS发病具有一定地域性和季节性,从事农务劳作的中老年群体为高危人群,DBV流行主要基因型为F型。
Abstract
Objective To analyze the etiological dectection results of severe fever with thrombocytopenia syndrome (SFTS) in Anqing City,and to provide basic data for the scientific prevention and control of SFTS. Methods RT-PCR was used to detect dabie bandavirus (DBV) in 502 serum samples of suspected SFTS cases,and the epidemiological information of confirmed cases was analyzed by SPSS 25.0.S gene sequencing was performed on some isolated virus strains to construct a phylogenetic tree. Results Among 502 samples of suspected SFTS cases,151 were positive for DBV RNA,with a positive rate of 30.08%.The ratio of the male positive rate (27.83%) to female positive rate (31.72%) was 0.88∶1.There was a significant difference in the positive rate among all age groups (χ2=15.271,P=0.033),and 71-80 years old group accounted for the highest proportion(38.84%).Farmers accounted for 84.77% of the total number of cases.The cases were distributed from April to October,and cases in May accounted for the highest proportion (27.82%).Taihu County had the highest proportion of cases (34.75%),and there were statistical differences in the positive rate among different countries (χ2=18.080,P=0.021).S fragments of 21 DBV strains were obtained,including 10 strains of the F genotype,5 strains of the A genotype,5 strains of the E genotype,and 1 strain of the B genotype. Conclusion In Anqing City and surrounding areas,the SFTS epidemic showed regional and seasonal distribution.Middle-aged and elderly people engaged in agricultural work are high-risk groups.The main genotype of DBV was F.
关键词
发热伴血小板减少综合征 /
大别班达病毒 /
基因分型
Key words
Severe fever with thrombocytopenia syndrome /
Dabie bandavirus /
Genotyping
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