目的 了解安徽省肠炎沙门菌耐药谱及其分子分型特征。方法 对安徽省 6个市2018—2021年分离培养的128株肠炎沙门菌进行药敏实验,采用脉冲场凝胶电泳(PFGE)进行分子分型,运用BioNumerics 6.6软件对PFGE图谱进行聚类分析。结果 128株肠炎沙门菌对14种抗生素产生不同程度耐药,耐药率最高的是萘啶酸(98.43%,126/128),其次是氨苄西林(87.50%,112/128)、氨苄西林/舒巴坦(69.53%,89/128)。128株肠炎沙门菌基因组切分为35种带型,各种带型间相似度为67.10%～100.00%。结论 安徽省6个市肠炎沙门菌耐药率较高,PFGE带型相似度较高,应加强传染源调查,规范抗生素使用。
Abstract
Objective To understand the drug resistance spectrum and molecular typing characteristics of Salmonella enteritis in Anhui Province. Methods 128 strains of Salmonella enteritidis isolated and cultured in 6 cities of Anhui Province from 2018 to 2021 were tested for drug sensitivity.The molecular typing was performed by pulsed field gel electrophoresis (PFGE),and the cluster analysis of PFGE patterns was performed by BioNumerics 6.6 software. Results The 128 strains of Salmonella enterica were resistant to 14 antibiotics to varying degrees,and the highest resistance rate was nalidixic acid (98.43%,126/128),followed by ampicillin (87.50%,112/128),and ampicillin/sulbactam (69.53%,89/128).The genomes of the 128 strains of Salmonella enterica were sectioned into 35 banding types,and the similarity among the various banding types ranged from 67.10% to 100.00%. Conclusion The drug resistance rate of Salmonella enteritidis was high in six cities in Anhui Province,and the similarity of PFGE banding was high,so the investigation of infectious sources should be strengthened and the use of antibiotics should be standardized.
关键词
沙门菌 /
耐药性 /
脉冲场凝胶电泳 /
分子分型
Key words
Salmonellae /
Drug resistance /
Pulsed-field gel electrophoresis /
Molecular typing
{{custom_sec.title}}
{{custom_sec.title}}
{{custom_sec.content}}
参考文献
[1] 许敬平,靳连群,冯华,等.沙门菌食物中毒的研究进展 [J].人民军医,2018,61(3):274-277.
[2] Xu Z,Song Q,Li C,et al.Characterization of ciprofloxacin-resistant and ESBL-producing Salmonella enteric serotype Derby in Eastern China[J].BMC Microbiol,2019,19(1):61.
[3] Nakao JH,Talkington D,Bopp CA,et al.Unusually high illness severity and short incubation periods in two foodborne outbreaks of Salmonella Heidelberg infections with potential coincident Staphylococcus aureus intoxication[J].Epidemiol Infect,2018,146(1):19-27.
[4] 张竹慧,陈晴晴,撒楠,等.2015—2020年安徽省腹泻病人沙门菌血清型分布及耐药性研究[J].中国食品卫生杂志,2021,33(5):536-541.
[5] 梁艳茹,陈诺,杨博雯,等.2020—2022年上海市浦东新区市售食品中沙门菌耐药特征研究[J].上海预防医学,2023,35(4):374-379.
[6] Wang Y,Zhang W.Clinical characteristics and drug resistance analysis of 90 cases of children with Salmonella enteritis[J].Comput Math Methods Med,2022,2022:5091945.
[7] 严伟,查赟峰,纪蕾,等.2018—2020年湖州市食源性疾病患者感染沙门菌耐药与分子分型研究[J].中国预防医学杂志,2022,23(8):622-627.
[8] 孙炀明,林云,罗建勇,等.2017—2019年嘉兴市食源性疾病监测结果分析[J].中国公共卫生管理,2021,37(2):217-219.
[9] 金迪,郭宝福,孙桂菊.南京市2018年食源性疾病主动监测流行病学特征[J].中国公共卫生,2021,37(3):564-567.
[10] 董晓璐,秦晓杰,刘阳泰,等.食源性沙门氏菌在人体胃肠道中耐受及致病机制研究进展[J].食品与发酵工业,2021,47(24):286-292.
[11] 姚玉芳,张竹慧,杨小双.黄山市43株沙门氏菌耐药分析及PFGE分子分型[J].安徽预防医学杂志,2022,28(3):202-205+211.
[12] 黄丽雯,李静媚,洪敏丽,等.一起肠炎沙门菌食物中毒的调查和检测分析[J].海峡预防医学杂志,2022,28(1):93-95.
[13] 王轶,宋秋坤,石岩,等.一起肠炎沙门菌食物中毒事件病原学分析[J].医学动物防制,2021,37(8):771-774.
[14] 王超.肠炎沙门氏菌耐药特征及PFGE分子分型研究[D].扬州:扬州大学,2018.
基金
国家科技重大专项(2018ZX10713003-002-005)
PDF(1202 KB)
