目的 了解宿州市2014年手足口病主要流行型别和分布特征,为制订手足口病防控方案及临床治疗提供实验室依据。方法 采用Real-time RT-PCR方法检测肠道病毒71型(EV71)、柯萨奇病毒A组16型(CoxA16)的特异性核酸。结果 343份手足口病临床诊断病例标本,肠道病毒核酸总阳性185份,阳性率59.2%。其中EV71阳性45份,阳性率13.1%;CoxA16阳性98份,阳性率28.6%;其他肠道病毒42份,阳性率12.2%。男女比为1.8:1,男女阳性率分别为54.6%(118/216)和52.8%(67/127)。结论 2014年宿州市手足口病主要流行型别为CoxA16,同时伴有EV71和其它肠道病毒;男女阳性检出率差异无统计学意义(χ²=0.113,P>0.05)。各县区阳性检出率差异有统计学意义(χ²=16.417,P<0.05)。

Objective To study the pathogens and distribution characteristics of hand-foot-mouth disease (HFMD) in Suzhou city in 2014, and provide scientific evidence for making effective measures. Methods The Real-time RT-PCR method was applied for Enterovirus 71 and Coxasckievirus A16 Nucleic acid detection. Results 185 cases of the 343 clinically diagnosed cases were positive. Positive rate was 59.2%. Of them, there were 45 cases infected with Enterovirus 71 and 98 cases infected with Coxasckievirus A16, with positive rate of 13.1% and 28.6%, respectively. The sex ratio was 1.8:1, and the positive rate of male and female was 54.6% and 52.8%, respectively. Conclusion CoxA16 was the major pathogens causing HFMD in Suzhou in 2014, accompanied by EV71 and other intestinal virus.There was no significant difference(χ²=0.113,P>0.05) in detection rate between male and female, however, the difference was significantly(χ²=16.417,P<0.05) among counties.