目的 建立石墨炉原子吸收光谱仪直接测定全血中锰操作简便、结果稳定的方法。 方法 通过优化石墨炉升温程序,选用不同基体改进剂,采取标准加入法直接检测血锰含量。结果 在适宜的测定条件下,该方法的检出限为0.5 μg/L,在1.0~20.0 μg/L范围,标准曲线相关系数可达0.9990,RSD1.8%~5.8%,加标回收率为90.0%~104.0%。结论 该方法操作简单,具有较高的灵敏度和准确度,快速简便,适用于血中锰的检测。

Objective To develop an easy and stable method of manganese determination in human blood by graphite furnace atomic absorption spectrometry (GFAAS). Methods By optimizing the experimental conditions of graphite temperature and proportions of matrix modifiers, the standard addition method was used to determine the manganese in blood. Results Under the optimal conditions, the determination limit for blood manganese was 0.5 μg/L. The absorbance showed linear to the manganese concentration in the range of 1.0~20.0 μg/L and the correlation coefficient was over 0.9990. Conclusion The method with high sensitivity and good accuracy is easy and rapid, which is suitable for determination of manganese in blood.