目的 了解蚌埠地区发热伴呼吸道症候群细菌性病原谱的构成特征。方法 按照科技重大专项监测技术方案收集患者痰液、咽拭子、血液、尿四份样本,痰液、血液进行金黄色葡萄球菌(SA)、肺炎克雷伯氏菌(KP)、铜绿假单胞菌(PA)、A组链球菌(GAS)、肺炎链球菌(SP)、流感嗜血杆菌(HI)、军团菌(LB)七种细菌的培养,培养阴性者再对标本进行上述七种病原菌和肺炎衣原体(CP)、肺炎支原体(MP)的多重荧光定量PCR核酸检测;咽拭子样本进行CP、MP的核酸检测;尿液标本用胶体金法进行SP及LB的特异抗原快速检测。结果 241份痰液样本细菌培养有1份样本分离出SA,痰液样本9种病原菌荧光PCR核酸检测,SP阳性率为19.09%、HI阳性率10.79% 、MP阳性率7.88%、KP阳性率6.64%、SA阳性率6.22%、PA阳性率5.39%、GAS阳性率2.9%、CP阳性率2.07% ,LB未检出; 268份血液样本培养全部阴性,核酸检测,HI阳性率1.49%,SA和PA均为1.13%; 269份咽拭子标本核酸检测CP阳性率2.6%、MP阳性率2.23%;267份尿标本检测结果KP阳性率0.75%、LB未检出。结论 蚌埠市发热伴呼吸道症候群患者病原菌以SP为主,其次是HI,MP、KP、SA、PA感染率水平相差不大,GAS和CP感染率较低。

Objective To study the composition of bacterial pathogens in patients with fever and respiratory tract syndrome in Bengbu city. Methods All samples of sputum, throat swabs, blood and urine were collected for each patient with fever and respiratory symptoms in accordance with the technical special monitoring program. Sputum and blood were collected for Staphylococcus aureus (SA), Klebsiella pneumoniae (KP), Pseudomonas aeruginosa (PA), Group A Streptococcus (GAS), Streptococcus pneumoniae (SP), Haemophilus influenzae (HI), Legionella bacteria (LB) culture. Multiple fluorescence quantitative PCR nucleic acid detection of the seven pathogenic bacteria and CP and MP of the specimens were carried out for the negative culture. The nucleic acid detection of CP and MP was detected in the swab samples, and the urine specimens were detected by colloidal gold method for the rapid detection of specific antigens of SP and LB. Results 1 case of SA was cultured in 241 samples of sputum . Detection of 9 pathogenic bacteria in sputum samples by fluorescence PCR nucleic acid. The positive rate of SP was 19.09%, HI positive rate was 10.79%, MP positive rate was 7.88%, KP positive rate was 6.64%, SA positive rate was 6.22%, PA positive rate was 5.39%, GAS positive rate was 2.9%, the positive rate of CP 2.07%, LB was not detected. Conclusion The pathogen of fever associated with respiratory tract syndrome in Bengbu city was mainly SP, followed by HI, MP, KP, SA and PA infection rate, GAS and CP infection rate were low.