目的 了解砀山县2015～2017年手足口病病原学分布及流行情况。方法 采集2015～2017年砀山县人民医院临床诊断为手足口病病例的咽拭子标本,共180份样本。采用荧光定量PCR( RT-PCR)方法进行肠道病毒71型(EV71)、柯萨奇病毒A16型(CoxA16)和肠道通用型核酸检测。结果 180份手足口病临床诊断病例标本,其中82份肠道病毒核酸阳性,阳性率45.56%。2015～2017年砀山县肠道病毒阳性率分别为46.67%、38.33%、51.67%,各年度阳性率差异无统计学意义(χ²=2.195.P=0.334)。EV71总阳性数18例,CoxA16阳性12例,其他肠道病毒52例,分别占21.95%、14.64%、63.42%,男女阳性率分别为46.96%(54/115)和43.07%(28/65)。结论 2015~2017年砀山县手足口病主要病原没有发生变化,三个年度均以其他肠道病毒为优势流行病原,同时伴有EV71和CoxA16感染。

Objective To understand the etiology and distribution of hand foot mouth disease (HFMD) in Dangshan county from 2015 to 2017. Methods A total of 180 samples of throat swabs were collected from People's Hospital of Dangshan County from 2015-2017. Fluorescent quantitative PCR (RT-PCR) was used to detect enterovirus 71 (EV71), coxsackievirus A16 (CoxA16) and intestinal universal nucleic acid. Results Of the 180 clinical specimens of HFMD, 82 were positive for enterovirus nucleic acid, with a positive rate of 45.56%. From 2015 to 2017, the positive rate of enterovirus was 46.67%, 38.33% and 51.67%, respectively. There was no significant difference in the positive rate of each year(χ²=2.195. P=0.334). There were 18 cases of EV71 positive, 12 CoxA16 positive and 52 other enterovirus, accounting for 21.95%, 14.64%, 63.42% respectively. The positive rates of male and female were 46.96% (54/115) and 43.07% (28/65), respectively. Conclusion From 2015 to 2017, the main epidemic pathogens of HFMD in Dangshan county were not changed, and other enteroviruses were the dominant pathogens in three years, accompanied by EV71 and CoxA16.