目的 了解纳米氧化钛暴露后卵巢中细胞分化相关基因的表达。方法 以ICR雌性小鼠为试验对象,暴露纳米氧化钛30 d、60 d、90 d后,分别处死小鼠,应用Illumina BeadChip 进行微阵列分析,研究纳米TiO2颗粒暴露90 d后卵巢细胞分化相关基因表达的改变。结果 ICR雌性小鼠暴露纳米TiO2颗粒后已知功能的细胞分化基因表达水平发生显著的改变,9个已知功能的细胞分化基因表达上调(diffscore≥13,P<0.05),5个已知功能的细胞分化基因表达下调(diffscore≥-13,P<0.05)。结论 纳米氧化钛暴露导致小鼠卵巢中细胞分化相关基因表达水平显著改变。
Abstract
Objective To investigate the gene expression related to cell differentiation in ovary after nano-titanium oxide exposure. Methods ICR female mice were used as experimental subjects. After exposure to nano-titanium oxide for 30, 60 and 90days, the mice were sacrificed respectively. Illumina BeadChip was used for microarray analysis to study the changes of gene expression related to ovarian cell differentiation 90 days after exposure to nano-titanium dioxide particles. Results After exposure to nano-titanium dioxide particles, the expression level of differentiated genes in ICR female mice with known functions changed significantly. The expression of nine differentiated genes with known functions was up-regulated (diffscore≥13,P<0.05), and the expression of five differentiated genes with known functions was down-regulated (diffscore≤-13,P< 0.05). Conclusion Exposure to nano-titanium oxide can significantly alter the expression level of differentiated genes in mouse ovaries.
关键词
纳米氧化钛 /
毒理试验 /
卵巢 /
细胞分化 /
基因表达
Key words
Nano-titanium oxide /
Toxicological test /
Ovary /
Cell differentiation /
Gene expression
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参考文献
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基金
江西省卫生计生委科技计划课题阶段成果,课题编号为20185390。
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