目的 通过分析2005~2018年安徽省肠出血性大肠杆菌O157∶H7监测资料,了解其在人群、家禽家畜感染情况和食品、水及媒介昆虫带菌情况。方法 2005~2018年分别在芜湖市、蚌埠市、萧县设立监测点,采集腹泻病人粪便、宿主动物粪便、媒介昆虫、食品等标本,经增菌、分离培养O157∶H7菌株,并用PCR 方法对阳性菌株进行毒力基因测定。结果 2005~2018年共监测各类标本21 966份,检出肠出血性大肠杆菌O157∶H7阳性标本123份,总阳性率为0.56%。其中宿主动物粪便阳性率最高,为1.07%(97/9 082);媒介昆虫标本阳性率为0.93%(17/1 835);食品、水标本阳性率为0.24%(6/2 533);腹泻病人粪便阳性率为0.04%(3/8 516)。从阳性标本中分离O157∶H7菌株123株,PCR测定2013~2018年检测出的菌株均未携带毒力基因。结论 安徽省监测点宿主动物仍有散在的动物感染肠出血性大肠杆菌O157∶H7,且萧县存在人感染O157∶H7病例,应继续加强监测和对动物粪便的管理工作。
Abstract
Objective To analyze the surveillance data of enterohemorrhagic Escherichia coli (EHEC) O157∶H7 from 2005 to 2018 in Anhui Province,and to understand the infection situation of EHEC O157∶H7 in human,poultry and livestock,and the carrier situation of food,water and vector insects. Methods From 2005 to 2018,monitoring points were set up in Wuhu City,Bengbu City and Xiaoxian County to collect diarrhea patients feces,host animals feces,vectors insects,food and other samples.O157∶H7 strain was isolated and cultured by enrichment,and virulence genes of the positive strain were determined by PCR. Results From 2005 to 2018,a total of 21966 samples were monitored and 123 strains of EHEC O157∶H7 were detected,with a total positive rate of 0.56%.Among them,the positive rate of host animal feces was the highest,which was 1.07% (97/9 082),the positive rate of vector insect samples was 0.93% (17/1 835),the positive rate of food and water samples was 0.24% (6/2 533),and feces were positive in 3 patients with diarrhea,the positive rate was 0.04% (3/8 516).123 strains of O157∶H7 were isolated from the positive samples.None of the positive strains detected by PCR from 2013 to 2018 carried virulence genes. Conclusion In Anhui Province,there were still scattered animals infected with EHEC O157∶H7 in the host animals,and there were human cases infected with O157∶H7 in Xiaoxian County.Monitoring and management of animal waste should continue to be strengthened.
关键词
肠出血性大肠杆菌O157∶H7 /
监测 /
宿主动物 /
新发传染病
Key words
Enterohemorrhagic Escherichia coli O157∶H7 /
Surveillance /
Host animals /
Emerging infectious diseases
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