目的 通过对安徽省一例人感染猪链球菌患者血液标本分离的菌株进行鉴定分型,了解菌株的分子特征。方法 采用Vitek2 compact全自动细菌鉴定仪对分离菌株进行生化鉴定,玻片凝集法进行血清型鉴定,PCR法检测菌株5种毒力基因和多位点序列分型(MLST),PubMLST数据库获得ST型别。结果 分离菌株经生化和血清型鉴定,确定为猪链球菌2型,毒力基因cps2J、sly、ef、gadph 的PCR检测结果均为阳性,mrp检测结果为阴性,MLST为ST7型。结论 分离病原菌为cps2J+/sly+/ef+/gadph+/mrp- ST7型猪链球菌2型,该型别人源猪链球菌在安徽省属首次报道,具有较强毒力,疫源地应加强防控,防止病例的再次出现。
Abstract
Objective To identify and classify a strain isolated from the blood of a patient infected with Streptococcussuis in Anhui Province,and to analyze its molecular characteristics. Methods Vitek 2 compact automatic bacterial identification instrument was used to identify the isolated strain biochemically,slide agglutination method was used to identify the serotype,PCR method was used to detect five virulence genes and multilocus sequence typing (MLST),and PubMLST database was used to obtain ST type. Results The isolated strain was identified as Streptococcussuis type 2 by biochemical and serotype identification.The PCR results of the virulence genes cps2J,sly,ef and gadph were all positive.The result of mrp was negative and MLST was ST7 type. Conclusion The isolated strain was Streptococcusuis type 2 (cps2J+/sly+/ef+/gadph+/mrp-ST7),which had strong virulence.The ST7 type strain from human was first reported in Anhui Province.Prevention and control measures should be strengthened in the epidemic foci to prevent the recurrence of cases.
关键词
猪链球菌 /
毒力基因 /
多位点序列分型
Key words
Streptocossus suis /
Virulence gene /
Multilocus sequence typing
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基金
艾滋病和病毒性肝炎等重大传染病防治(项目号:2018ZX10713003-002-005)
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