目的 了解安庆市无偿献血者中人类嗜T淋巴细胞病毒(HTLV-I/II)感染情况,为制定血液筛查策略提供依据,确保血液质量安全。方法 用 ELISA 法筛查无偿献血者血浆中抗-HTLV-I/II,筛查呈阳性的标本进行双孔复检,复试阳性标本送国家临床检验中心用WB和实时荧光 PCR 法进行确证实验。结果 30 457份无偿献血者标本中,初筛出17份阳性标本,阳性率为0.06%,经PCR和WB法确认,17份结果全阴性。结论 安庆市无偿献血者人群属于 HTLV 感染的低发区或非流行区。安庆市无需开展常规筛查。
Abstract
Objective To investigate the infection of human T-lymphotropic virus type I or type II (HTLV-I / II) in blood donors in Anqing City,and to provide basis for making blood screening strategy and ensure blood quality and safety. Methods Anti HTLV-I / II in the plasma of the voluntary blood donors was screened by ELISA,and the positive samples were retested by two holes.The positive samples were sent to the national clinical examination center for confirmation by WB and real-time PCR. Results Of the 30457 blood donor specimens,17 positive specimens were initially screened,with a positive rate of 0.06%,and confirmed by PCR and WB methods,17 results were all negative. Conclusion The voluntary blood donors in Anqing City belonged to the low incidence or non epidemic area of HTLV infection.There was no need for routine screening in Anqing City.
关键词
无偿献血者 /
人类嗜T淋巴细胞病毒 /
感染筛查
Key words
Voluntary blood donors /
Human T- lymphocyte virus /
Infection Screening
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