目的 了解2018年安徽省一起感染性胃肠炎暴发疫情的病原学特征。方法 收集2018年10月一起急性胃肠炎病例粪便/肛拭子12份标本,采用Real-time PCR检测诺如病毒GII基因组,阳性标本经RT-PCR扩增聚合酶区和衣壳蛋白区部分基因序列,进行核苷酸序列测定和遗传进化树分析。结果 12份急性胃肠炎病例标本,荧光PCR检出诺如病毒核酸均为阳性,测序获得10条序列。遗传进化树显示:10条 GII.2型间核苷酸序列同源性为100%,与台州参考株GII.2(GenBank登录号:MH806429,MH842206)同源性最高(同源性最高为99.47%),且处于同一分支。结论 此次急性胃肠炎暴发疫情由GII.P16-GII.2诺如病毒引起,鉴于它在中国的广泛流行,应重视GII.P16-GII.2重组株在本地区诺如病毒的监测。
Abstract
Objective To identify the genotypes and molecular characteristic of an acute gastroenteritis outbreak in Anhui province in 2018. Methods Facal/anal swab samples collected from this outbreak and sporadic cases were detected by Real-time fluorescent quantitative PCR.Partial RNA dependent RNA Polymerase(RdRp) region and capsid region nucleotides of GII NoV positive samples were amplified by RT-PCR.All samples were sequencedand analyzed. Results Twelve fecal/ anal swab specimens from the patients were posotive.Ten posotive samples were sequenced successfully.The homolgous and phylogenetic analysis showed that the isolates belonged to norovirus type GII.2. They have high homology in sequences of nucleotide and amino acid respectively with the strain from Taizhou China(MH806429,MH842206). Conclusion The acute gastroenteritis was caused by GII.P16-GII.2 norovirus, considering that it had already triggered epidemic in China, the monitoring of this novel GII.P16-GII.2 variant will be a crucial part of NoV surveillance in the future.
关键词
急性胃肠炎 /
诺如病毒 /
暴发疫情 /
基因型 /
分子生物学
Key words
Acute gastroenteritis /
Norovirus /
Outbreak /
Genotype /
Molecular biology
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参考文献
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基金
安徽省卫生计生委科研计划项目(项目号:2017jk003)
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