目的 回顾分析2020年芜湖市新型布尼亚病毒(SFTSV)哨点监测结果,了解其基因型分布情况,为芜湖市新型布尼亚病毒疫情防控与研究提供基础资料。方法 通过实时荧光定量 RT-PCR的方法检测108例疑似发热伴血小板减少综合征(SFTS)病例血清中的SFTSV核酸,采用RT-PCR方法扩增核酸阳性标本中SFTSV的S基因片段,测其序列并构建系统进化树分析基因型。结果 108例疑似病例样本中检出24例SFTSV核酸阳性。病例时间分布均为4—10月,其中6月份检出最多(10例),占41.67%;发病年龄集中在60岁以上老年人(18例),占75.00%;病例职业以农民为主(22例),占91.67%。S基因片段测序成功14例,成功率58.30%,其中C2基因型6例、C3基因型1例、C4基因型4例、J基因型3例。结论 芜湖市及周边地区新型布尼亚病毒感染有较明显的年龄、职业、季节性分布特点,基因型以C2基因型为主,C3、C4和J基因型也有不同程度的分布。
Abstract
Objective To review and analyze the sentinel surveillance results of severe fever with thrombocytopenia syndrome bunyavirus (SFTSV) in Wuhu City in 2020,and to understand its genotype distribution,so as to provide basic data for the prevention,control and research of SFTSV in Wuhu City. Methods The SFTSV nucleic acid in the serum of 108 suspected cases of severe fever with thrombocytopenia syndrome (SFTS) was detected by real time fluorescence quantitative RT-PCR.Then RT-PCR was used to amplify the S gene fragment from SFTSV nucleic acid positive specimens,and its sequence was measured and phylogenetic tree was constructed to analyze the genotype. Results Among 108 suspected cases,24 cases were found to be positive for the nucleic acid of SFTSV.The distribution time of all cases was from April to October,and the maximum number of cases was 10 in June (41.67%).The onset age was concentrated in the elderly over 60 years old,with 18 cases accounting for 75.00%.Farmers were the main occupation of the patients,with 22 cases accounting for 91.67%.Among the 24 positive specimens,14 cases were successfully sequenced,with a success rate of 58.30%.There were 6 cases of C2 genotype,one case of C3 genotype,4 cases of C4 genotype and 3 cases of J genotype. Conclusion The SFTSV infection in Wuhu City and its surrounding areas has obvious distribution characteristics of age,occupation and seasonality.The genotype is mainly C2 genotype.C3,C4 and J genotypes are also distributed in different degrees.
关键词
发热伴血小板减少综合征 /
新型布尼亚病毒 /
基因型
Key words
Sever fever with thrombocytopnia syndrome /
Severe fever with thrombocytopenia syndrome bunyavirus /
Genotype
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参考文献
[1] Yu XJ,Liang MF,Zhang SY,et al.Fever with thrombocytopenia associated with a novel bunyavirus in China[J].N Engl J Med,2011,364(16):1523-1532.
[2] 孙永,张永根,胡万富,等.新型布尼亚病毒安徽分离株全基因组测序分析[J].安徽预防医学杂志,2013,19(6):403-405.
[3] Zhan J,Wang Q,Cheng J,et al.Current status of severe fever with thrombocytopenia syndrome in China[J].Virol Sin,2017,32(1):51.
[4] 朱守俊,胡玉兰,郭婷婷,等.46例新型布尼亚病毒致发热伴血小板减少综合征临床分析[J].安徽医专学报,2021,20(4):28-30.
[5] Cui F,Cao HX,Wang L,et al.Clinical and epidemiological study on severe fever with thrombocytopenia syndrome in Yiyuan County,Shandong Province,China[J].Am J Trop Med Hyg,2013,88(3):510-512.
[6] Bao CJ,Guo XL ,Qi X ,et al.A family cluster of infections by a newly recognized bunyavirus in eastern China,2007:further evidence of person-to-person transmission[J].Clin Infect Dis,2011,53(12):1208-1214.
[7] Liu Y,Li Q,Hu W,et al.Person-to-person transmission of severe fever with thrombocytopenia syndrome virus[J].Vector Borne Zoonotic Dis,2012,12(2):156-160.
[8] 魏艳艳,邹桂舟,叶珺,等.新型布尼亚病毒感染致发热伴血小板减少综合征临床特点及血常规和血清酶学分析[J].安徽医药,2016,20(5):903-907.
[9] 马涛,徐庆,李晨,等.2010—2016年南京市发热伴血小板减少综合征流行特征分析[J].现代预防医学,2017,44(16):2890-2894.
[10] 何益新,黄永,何嗣胜,等.铜陵市一起发热伴血小板减少综合征聚集性疫情流行病学调查研究[J].中国预防医学杂志,2018,19(7):553-556.
[11] 伍婷,柳燕,李鸿儒,等.巢湖地区新布尼亚病毒感染患者的临床特点和病重相关危险因素分析[J].中华疾病控制杂志,2017,21(1):56-60.
[12] 宋玉亮,叶继斌,熊晨晖,等.一种新布尼亚病毒快速检测方法的建立及应用[J].中华检验医学杂志,2012,35(2):180-182.
[13] 吕燕宁,王全意,窦相峰,等.发热伴血小板减少综合征布尼亚病毒实时荧光RT-PCR检测方法的建立[J].国际病毒学杂志,2011,18(4):111-115.
[14] Zhang Y,He YW,Dai YA,et al.Hemorrhagic fever caused by a novel Bunyavirus in China :pathogenesis and correlates of fatal outcome[J].Clin Infect Dis,2012,54(4):527-533.
[15] 迟媛媛,翟慎勇,温红玲,等.发热伴血小板减少综合征患者血清中新型布尼亚病毒RNA的检测[J].山东大学学报(医学版),2012,50(11):118-121.
[16] 刘秀兰,姚学君,张雪峰,等.我国部分新型布尼亚病毒分离株全基因组序列分析[J].江苏预防医学,2018,29(6):623-625,648.
[17] 董梅,姚学君,马永法.江苏省新型布尼亚病毒分离株全长S片段基因特征分析[J].预防医学,2019,31(1):65-67.
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