目的 了解芜湖市腹泻患者沙门菌血清型、分子分型分布特征及耐药情况,为沙门菌监测提供依据。方法 对芜湖市腹泻患者粪便中分离的40株沙门菌进行血清分型和脉冲场凝胶电泳(PFGE)分子分型分析并运用BioNumeries 6.6 软件进行聚类分析,采用革兰氏阴性菌药敏检测板进行细菌耐药性检测。结果 40株沙门菌有7个血清群、11种血清型,最多的是D群肠炎沙门菌(14株),其次是B群鼠伤寒沙门菌(12株)和C1群蒙得维的亚沙门菌(5株)。经PFGE分型40株沙门菌共分成30个带型,带型同源性45.80%~100.00%,14株肠炎沙门菌带型有6种,12株鼠伤寒沙门菌带型有12种,血清型相同的沙门菌PFGE带型有同源性。肠炎沙门菌、里森沙门菌有成簇现象。40株沙门菌对萘啶酸耐药率最高,为60.00%,其次为氨苄西林、链霉素、四环素,多重耐药率达62.50%。结论 芜湖市腹泻患者分离的沙门菌多种血清型和基因型同时流行,存在潜在暴发风险,耐药形势严峻,需加强对抗生素使用的监管力度,减少耐药株的出现和传播。
Abstract
Objective To understand the serotype,molecular type distribution and drug resistance of Salmonella from patients with diarrhea in Wuhu City,and to provide the basis for Salmonella surveillance. Methods Serotyping and molecular typing analysis by pulsed-field gel electrophoresis (PFGE) were performed on 40 strains of Salmonella isolated from feces of diarrhea patients in Wuhu City during.The PFGE typing results were cluster analyzed by BioNumeries 6.6 software.The bacteria′s antimicrobial resistance was detected using a gram-negative drug susceptibility test plate. Results There were 7 serogroups and 11 serotypes in the 40 isolates,the most of which were 14 strains of Salmonella enteritidis in group D,12 strains of Salmonella typhimurium in group B and 5 strains of Salmonella Montevideo in group C1.The 40 strains of Salmonella were classified into 30 band types by PFGE,and the band type homology was 45.80%-100%.There were 6 band types of 14 strains of Salmonella enteritidis and 12 band types of 12 strains of Salmonella typhimurium.The PFGE band types of Salmonella with the same serotype were homologous.There were clusters of Salmonella enteritidis and Salmonella rishonensis.The drug resistance rate of 40 Salmonella strains to nalidixic acid was 60.00%,followed by ampicillin,streptomycin,tetracycline,and the multiple drug resistance rate reached 62.50%. Conclusion Multiple serotypes and genotypes of Salmonella isolated from diarrhea patients in Wuhu City are prevalent simultaneously,with potential outbreak risk and serious drug resistance situation.Antibiotic use management and supervision should be strengthened to reduce the emergence and spread of drug-resistant strains.
关键词
沙门菌 /
血清分型 /
PFGE分型 /
药敏
Key words
Foodborne diseases /
Salmonella /
Serotyping /
PFGE typing /
Drug sensitivity
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参考文献
[1] Olsen SJ,Bishop R,Brenner FW,et al.The changing epidemiology of salmonella: trends in serotypes isolated from humans in the United States,1987-1997[J].J Infect Dis,2001,183(5):753-761.
[2] 杨柳青,欧新华,贾华云,等.长沙市沙门菌表型特征及PFGE分子分型[J].实用预防医学,2016,23(1):39-42.
[3] 杜银菊,梁胜楠,段瑶,等.2015—2017年聊城市腹泻病例中沙门氏菌分子分型及耐药性研究[J].中国人兽共患病学报,2019,35(10):915-921.
[4] 李娜,刘辉,李健,等.济南市腹泻患者沙门氏菌PFGE分子分型及耐药特征研究[J].中国人兽共患病学报,2021,37(10):893-897+902.
[5] 王丽丽,徐建国.脉冲场凝胶电泳技术(PFGE)在分子分型中的应用现状[J].疾病监测,2006,21(5):276-279.
[6] 石亚锋,张建群,符凌辉,等.2015—2020年浙江省余姚市沙门菌感染流行特征分析[J].疾病监测,2023,38(2):185-190.
[7] 姚玉芳,张竹慧,杨小双.黄山市43株沙门氏菌耐药分析及PFGE分子分型[J].安徽预防医学杂志,2022,28(3):202-205+211.
[8] 洪颖,王利,陈谨,等.2010—2018年安徽省马鞍山市腹泻患者中非伤寒沙门菌的分子分型与耐药性研究[J].中国人兽共患病学报,2020,36(8):646-653.
[9] Talon D,Cailleaux V,Thouverez M,et al.Discriminatory power and usefulness of pulsed-field gel electrophoresis in epidemiological studies of Pseudomonas aeruginosa[J].J Hosp Infect,1996,32(2):135-145.
[10] 娄静,刁保卫,李杰,等.沙门菌脉冲场凝胶电泳分型与血清型的对应关系[J].中华流行病学杂志,2013,34(6):618-621.
[11] 刘伟,尹可欣,白婧,等.北京市海淀区食源性疾病沙门菌血清型分布和耐药性分析[J].中国卫生检验杂志,2022,32(1):64-67.
[12] 孙雅娜,刘坚龄,梁广忠,等.2018—2020年天津市津南区食源性致病菌分布及药敏分析[J].中国食品卫生杂志,2021,33(5):548-552.
[13] 苏良,杨柳青,宋迎春,等.2015—2019年长沙市食源性疾病沙门菌血清分型和耐药情况分析[J].实用预防医学,2022,29(1):82-84.
[14] 王炜,金萍,王燕,等.南京市2019年哨点医院腹泻重点病原菌药敏监测结果分析[J].医学动物防制,2022,38(9):874-877.
[15] 刘雪杰,陈伟伟,傅祎欣,等.2015—2018年福建省食源性疾病沙门氏菌监测情况分析[J].中国人兽共患病学报,2020,36(3):223-228.
[16] 胡凤清,叶素贞,饶晓虹,等.南平市零售生禽肉中沙门菌检出率、耐药性与分子特征分析[J].海峡预防医学杂志,2022,28(3):44-47.
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