目的 探索建立测定母乳中碘含量的前处理和检测方法。方法 母乳样品经HNO₃-H₂O₂体系微波消解后,用砷铈催化分光光度法测定消解液中的碘含量。结果 乳汁碘浓度在0～300 μg/L范围内相关系数大于0.9999,最低检测浓度为8 μg/L(取样量5.00 ml);高、中、低三种浓度相对标准差(RSD)<4%;回收率为86.5%～102.6%, 900份母乳碘含量测定结果平均值为169.2 μg/L。结论 微波消解-砷铈催化分光光度法测定母乳中碘含量,该方法具有灵敏度高、准确性好、重现性好等特点,适用于母乳中碘含量的测定。

Objective To explore and establish the pretreatment and determination of iodine in breast milk. Methods The breast milk samples were digested by a mixture of concentrated nitric acid and hydrogen peroxide (HNO₃- H₂O₂ ) in the sealed vessel, then the iodine in digestion solution was subjected to the analysis by arsenic cerium catalytic spectrophotometry. Results The standard curve range of iodine concentration was 0-300 μg/L, the correlation coefficient of standard curve was more than 0.9999,the minimum detectable concentration was 8 μg/L (Sampling volume of 5.00 ml), the recovery of standard addition was between 86.5% and 102.6%, and the relative standard deviation of high, medium and low concentration was less than 4%. The average iodine of 900 breast milk was 169.2 μg/L. Conclusion The method of microwave digestion-arsenic cerium catalytic spectrophotometry determination of iodine in breast milk could digest the sample thoroughly and this method had good reproducibility, high sensitivity, and good accuracy, suitable for the determination of iodine in breast milk.