目的 对安庆市某中学暴发的急性胃肠炎疫情进行病原鉴定,并进一步对部分基因序列测序分析,以确定其基因亚型。方法 对采集的13份肛拭子,3份生活饮用水标本,采用实时荧光定量RT-PCR方法进行诺如病毒GI/GII型检测,对阳性标本的病毒VP1区部分核酸序列测序和基因库数据比对,并采用MAGA5.0软件构建进化树。结果 在13份肛拭子样本7份诺如病毒阳性中,6份属于GI.3型,1份属于GII.17型;3份生活饮用水样本GI.3和GII.17型均有检出。结论 该起疫情由诺如病毒感染引起,为GI.3和GII.17基因亚型。
Abstract
Objective To identify the pathogen outbreaks of acute gastroenteritis in a middle school of Anqing city, and further sequencing of partial gene sequences was used to determine the gene subtype. Methods 13 anal swabs and 3 drinking water samples were detected by fluorescence quantitative PCR method for detection of norovirus type GI/GII. The sequence of positive strains nucleic acid VP1 region were amplified, sequenced and compared with the gene database, and the phylogenetic tree was constructed by MAGA5.0 software. Results In the 13 samples, 6 samples belonged to type GI.3, 7 belonged to GII.17 type. 3 copies of drinking water samples were detected by GI.3 and GII.17. Conclusion The epidemic was caused by norovirus GI.3 and GII.17 genotypes.
关键词
诺如病毒 /
急性胃肠炎 /
暴发疫情 /
基因亚型 /
基因突变 /
分子生物学
Key words
Noroviruses /
Acute gastroenteritis /
Outbreak /
Gene subtype /
Gene mutation /
Molecular biology
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