Objective To analyze the drug resistance of multidrug-resistant organisms (MDROs) and to provide basis for the rational clinical application of antibiotics. Methods 19 198 biological specimens were collected from different clinical departments of Fuyang People's Hospital in 2018. The specimens were isolated and cultured,and the strains were obtained and tested for drug susceptibility. Results 19 198 samples were collected in 2018 and 5605 strains of pathogenic bacteria were isolated,of which 1456 strains showed multidrug resistant,and the detection rate of multidrug-resistant organisms was 25.98%. The main resistant strains were Escherichia coli,Klebsiella pneumoniae,Acinetobacter baumannii,Staphylococcus aureus and Pseudomonas aeruginosa. Multidrug-resistant organisms were detected from sputum accounted for 50.07%. The drug resistance rate of Escherichia coli to ceftriaxone,levofloxacin and compound sulfamethoxazole was over 73.00%,Klebsiella pneumoniae to ceftriaxone was as high as 95.25%,and Staphylococcus aureus to oxacillin and penicillin was over 80%. Conclusion The high resistance rate of multidrug-resistant organisms to conventional antibiotics increased the difficulty of clinical treatment. It’s necessary to strengthen the monitoring of drug-resistant organisms and rational use of antibiotics.