Objective To understand infection status of human immunodeficiency virus(HIV), syphilis and hepatitis C virus(HCV) and the trends in high- risk behaviors among drug users and male patients in STD clinic. Methods According to the requirements of the national sentinel surveillance program, HIV, syphilis and HCV serological tests were carried out for drug users and male STD clinic patients from 2016 to 2018. Results From 2016 to 2018, a total of 1201 drug users and 1202 male STD clinic patients were monitored.The positive rates of HIV, syphilis and HCV in drug users were 0.25%, 2.66% and 54.87%, respectively.The positive rates of HIV, syphilis and HCV in male STD clinic patients were 0.42%, 5.57% and 0.0%,respectively.The positive rates of syphilis antibody and HCV antibody in drug users were higher than those in STD clinic men, the difference was statistically significant(χ²=12.875,P＜0.001; χ²=908.770,P＜0.001).The total awareness rate of AIDS related knowledge among drug users and STD clinic men was 91.34% and 57.15%, respectively, with statistical significance(χ²=367.119,P＜0.001). Conclusion In order to effectively control the spread and prevalence of AIDS, we should strengthen the health education of high-risk groups, increase the intervention of high-risk behaviors and other measures.