Objective To understand the status and related factors of perimenopausal syndrome among female staffs in municipal hospitals in Anhui Province. Methods A total of 895 female hospital staffs aged 40~60 were selected as research objects from Suzhou,Chuzhou and Chizhou by stratified cluster sampling method. Modified Kupperman Scale and Pittsburgh Sleep Quality Index (PSQI) were used to conduct the survey. Results Among 895 survey objects,419 suffered from perimenopausal syndrome with an incidence of 46.8%. The top three common symptoms were: fatigue,Arthralgia/myalgia,and irritability. Multivariate logistic regression analysis showed that Huaibei area (OR=1.964,95CI:1.335~2.890),more pregnancies (OR=1.517,95CI:1.059~2.175),menstrual disorders (OR=2.443,95CI:1.463~4.082) or amenorrhea (OR=2.975,95CI:1.929~4.588),and poor sleep quality (OR=6.702,95CI:4.44~10.106) were positively associated with the incidence of perimenopausal syndrome among female staffs in municipal hospitals,while drink milk regularly (OR=0.659,95CI:0.483~0.898) were negatively associated with the incidence of perimenopausal syndrome among female staffs in municipal hospitals. Conclusion The incidence of perimenopausal syndrome among female staffs in municipal hospitals aged 40 to 60 years was 46.8%. Area,number of pregnancies,menstrual conditions,milk consumption,and sleep quality were the related factors that affecting perimenopausal syndrome.