Objective To understand the disinfection status of nursery institutions within the jurisdiction of Jinghu District in Wuhu City,improve and strengthen the disinfection quality,and effectively control the occurrence of infectious diseases in nursery institutions. Methods From 2015 to 2019,15 kindergartens (Times),36 (Times),19 (Times),20 (Times) and 16 (Times) kindergartens were monitored respectively.The methods of field sampling and microbiological detection were used to detect and analyze 636 samples collected in 5 years.The disinfection effect of indoor air (static state),object surface,toys,staff hands,children's hands and tableware (drinking) in kindergartens was carried out monitoring. Results From 2015 to 2019,106 nursery institutions in Jinghu District of Wuhu City were monitored and 636 samples were tested,463 of which were qualified,and the qualified rate was 72.80%.The qualified rate had fluctuated in the past five years,and the qualified rate in 2017 was the lowest (55.26%).The difference was statistically significant in different years (χ²=36.212,P＜0.001).The qualified rate of different types of samples showed that the qualified rate of indoor air was the highest (97.17%),and the lowest was children's hand (46.23%),the difference in different types of samples was statistically significant (χ²=127.241,P＜0.001).The qualified rate of hands of children in public nursery institutions was significantly higher than that in private nursery institutions (χ²＝3.939,P＜0.05). Conclusion The disinfection quality of kindergartens in Jinghu District of Wuhu City in five years is unstable,and the qualified rate of object surface,staff hands and children's hands is relatively low.It is necessary to further strengthen the management and improve the disinfection quality of nurseries and kindergartens in the area.