Objective To develop a simple method of assaying microdose iodine in the fingernails. Methods The samples were digested by perchloric acid and potassium perchlorate and the iodine content of the sample was determined by arsenic cerium catalytic spectrophotometry.The linearity and liner near range of the standard curve,detection limit,precision,recovery rate of the newly developed arsenic cerium catalytic spectrophotometry were tested. Results The linear range of this method was 0~200μg/L,|R|≥0.9999.The detection limit is 1.29μg/g and the quantification limit is 4.30μg/g (sample 0.3ml).The inter assay coefficient of variation (CV)for one sample was 0.84%.The nails of the high,medium,and low iodine content groups were added sample and recovered.The adding sample amount was 2ng,15ng,and 20ng respectively.and the recovery rate was 105.4%. Conclsion The method has good precision and accuracy.The experimental method is simple and easy to operate.It can be used as a detection method of micro iodine in human nails.
Key words
Iodine /
fingernail /
Arsenic cerium catalytic spectro-photometry
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