Objective To understand the distribution of avian influenza virus (AIV) in the environment of Wenjiang District,and to provide reference for the formulation of prevention and control measures of avian influenza. Methods From 2017 to 2019,samples were collected from farmers' markets,mobile vendors,farmers,poultry point killing restaurants and other places in Wenjiang District.Real time fluorescent quantitative PCR was used to detect the nucleic acid of avian influenza A and identify subtypes H5,H7 and H9. Results A total of 465 samples were collected from 2017 to 2019,of which 101 were positive,with a total positive rate of 21.72%.The positive rate of avian influenza virus nucleic acid in 2019 was 38.33%,significantly higher than that in 2017 (9.21%) and 2018 (21.24%),and the difference was statistically significant (χ²= 33.490,P＜ 0.05).The positive rate of point killing catering environment in 2017 was 44.45%,significantly higher than that in other places(χ²=12.851,P＜0.05).The positive rate of mobile vendors in 2019 was 75%,significantly higher than that in other places (χ²=12.846,P＜0.05).The positive rate of cages in 2018 was 35.39%,significantly higher than that of other types of specimens (χ²=17.466,P＜0.05). Conclusion Avian influenza virus widely exists in the relevant external environment of Wenjiang District,and there is a risk of human infection of avian influenza in Wenjiang District.