Objective To analyze the distribution characteristics and the antimicrobial resistance of main isolates in a hospital,so as to provide the reference for reasonable use of antibacterial drugs in clinical departments. Methods A total of 5916 strains of common pathogenic bacteria were collected from clinical isolates in 2018.VITEK-2 compact system was used for bacterial identification,Mic method and K-B disk diffusion method were used for drug sensitivity test.WHONET 5.6 software was used for statistical analysis of the data. Results The specimens of 5916 strains were main originated from urine,sputum,secretions,and blood.The Gram-negative bacteria accounted for 66.9%,which mainly includes Escherichia coli.The Gram-positive bacteria accounted for 20.4%,which mainly includes Staphylococcus aureus.The Gram-negative bacteria such as Escherichia coli and Klebsiella pneumoniae have the highest resistance rates to ampicillin (82.8% and 71.2% respectively),and their resistance rates to carbapenems are 1.9% and 16.5% respectively.The Gram-positive bacteria such as Staphylococcus aureus and Staphylococcus epidermidis have the highest resistance rates to penicillin G (92.2% and 93.9% respectively),and their resistance rates to linezolid,vancomycin,and tigecycline are 0%.The resistance rates of Acinetobacter baumannii to imipenem and meropenem were 53.5% and 50.3% respectively.The resistance rates of Pseudomonas aeruginosa to imipenem and meropenem were 13.2% and 8.7% respectively. Conclusions There are many types of drug-resistant strains isolated from clinical in a hospital in 2018.Medical staffs should strictly implement the control measures of infection,and reasonably use antibacterial drugs.