Objective To analyze iodine nutritional status of children and pregnant women in areas with high water iodine and non-high iodine in Huainan City,and to provide scientific basis for classified guidance and scientific supplementation of iodine. Methods In non-iodine-rich counties and districts of the city,five townships were selected from each county area according to the east,west,north and south.Five administrative villages were selected from each county by systematic sampling method in high iodine areas.40 non boarding students aged 8-10 years and 20 pregnant women were selected from each selected township (Village).Urine samples and salt samples were collected for detection,and thyroid volume of students was detected by B-ultrasound. Results The qualified iodized salt consumption rate was 97.33%,the median urinary iodine of children was 244.25 μg/L,the goiter rate of children was 0.29%,and the median urinary iodine of pregnant women was 171.8 μg/L.The coverage rate of iodized salt was 100%,the median urinary iodine of children was 339.8 μg/L,the goiter rate of children was 1.43%,and the median urinary iodine of pregnant women was 203.3 μg/L. Conclusion The iodine deficiency disorders in non-iodine-rich areas of Huainan City are continuously eliminated.The iodine nutrition of children in non-iodine-excess areas is larger than the appropriate range and the iodine nutrition of pregnant women is at the appropriate level.The iodine nutrition of children in high water iodine area is excessive,and that of pregnant women is suitable.The strategy of prevention and control should be carried out according to local conditions,classified guidance and scientific iodine supplement.