Objective To understand the disinfection efficacy of a third-class hospital in Anhui Province,find the weak links and to scientifically guide the disinfection work. Methods Using field sampling and microbial culture to monitor sterilized articles,sterilized articles,disinfectants,endoscopes,dialysates,object surfaces,hand hygiene of medical staff and indoor air from January 2014 to December 2018,and the monitoring results were statistically analyzed. Results A total of 10094 samples were sampled from 2014 to 2018,9517 were qualified,and the overall qualified rate was 94.3%.The annual qualified rates were 93.8%,96.7%,92.7%,93.9% and 94.6% respectively,and the total qualified rate of type I environment was 95.2%,which was higher than that of other environments.In five years,the qualified rates of sterilized articles,disinfected articles,disinfectants,endoscopes,dialysate,object surfaces,hand hygiene and air were 97.6%,91.7%,98.5%,99.5%,93.8%,89.7%,90.2% and 97.7%,respectively. Conclusion The effect of disinfection and sterilization in hospital is generally well,but the qualified rate of object surface and hand hygiene is relatively low,so it is necessary to strengthen management to reduce the risk of nosocomial infection.