Molecular characteristics of pathogens in an outbreak of acute gastroenteritis

JIN Jing; SHI Yong-lin; ZHANG Wen-yan; Tang Ren-shu; ZHOU Wei; LIU Wei; ZHU Yin-di

doi:10.19837/j.cnki.ahyf.2021.02.006

Anhui Journal of Preventive Medicine ›› 2021, Vol. 27 ›› Issue (2) : 109-111. DOI: 10.19837/j.cnki.ahyf.2021.02.006

Molecular characteristics of pathogens in an outbreak of acute gastroenteritis

JIN Jing^*, SHI Yong-lin, ZHANG Wen-yan, Tang Ren-shu, ZHOU Wei, LIU Wei, ZHU Yin-di

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Abstract

Objective To investigate the pathogenic molecular characteristics of an acute gastroenteritis epidemic in Hefei City. Methods The nucleic acids of sapovirus,norovirus and astrovirus were detected in 13 anal swab specimens by Real-time PCR.The detected sapovirus nucleic acid positive specimens were used traditional RT-PCR to amplify the VP1 gene fragment,and the gene sequence was determined to construct a phylogenetic tree. Results The results of real-time PCR showed that 8 samples of sapovirus nucleic acid were positive,while norovirus and astrovirus nucleic acid were negative.One RT-PCR amplification product was sequenced successfully.Sequence alignment and evolutionary analysis showed that it was a subtype of sapovirus GII.3. Conclusions The pathogen causing the outbreak of acute gastroenteritis was sapovirus GII.3 genotype.

Key words

Sapovirus / Acute gastroenteritis / Evolutionary analysis / GII.3 genotype

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JIN Jing, SHI Yong-lin, ZHANG Wen-yan, Tang Ren-shu, ZHOU Wei, LIU Wei, ZHU Yin-di. Molecular characteristics of pathogens in an outbreak of acute gastroenteritis[J]. Anhui Journal of Preventive Medicine. 2021, 27(2): 109-111 https://doi.org/10.19837/j.cnki.ahyf.2021.02.006

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