Objective To investigate the serogroups,virulence genes and drug resistances characteristics of vibro parahaemolyticus in Hefei City from 2014 to 2019.So as to provide scientific basis for the prevention and control of vibrio parahaemolyticus infection. Methods 107 strains of vibro parahaemolyticus were grouped by slide agglutination method,.TLH,TDH and TRH virulence genes were detected by real-time PCR.And drug sensitivity was tested by K-B method. Results The 107 strains of vibrio parahaemolyticus belonged to 6 serogroups.Group O3 was the main serogroup,accounting for 83.2% (89 / 107),followed by group O4,accounting for 13.1% (14 / 107).The detection rates of TLH,TDH and TRH virulence genes were 100% (107 / 107),99.1% (106 / 107) and 0 (0 / 107),respectively.The resistance rates of 107 strains of vibrio parahaemolyticus to ampicillin and cefazolin were high,reaching 84.1% (90 / 107) and 82.2% (88 / 107),respectively.The drug resistance rate to cefoxitin,ceftazidime and cefepime was 0.9% (1 / 107),and it was completely sensitive to piperacillin,norfloxacin,levofloxacin,amikacin,gentamicin,tobramycin,trimethoprim /sulfaisoxazole,tetracycline,chloramphenicol and nitrofurantoin. Conclusion In recent years,the main clinical isolates of vibrio parahaemolyticus in Hefei City are O3 group,the TDH virulence gene carrying rate is very high,and the drug resistance of strains exists,so clinical medication should be standardized.
Key words
Vibrio parahaemolyticus /
Serogroup /
Virulence gene /
Drug resistance
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References
[1] 孙永,张竹慧,袁媛,等.2015年安徽地区副溶血性弧菌生物学特征及分子分型溯源分析[J].中国病原生物学杂志,2017,12 (4):317-320.
[2] 张晶,龚玉娇,陈佳璇,等.广州地区70株副溶血性弧菌病原学特征分析[J].现代预防医学,2018,45(4):704-707,719.
[3] 陈洪友,盛跃颖,屠丽红,等.2012年上海地区副溶血性弧菌血清分型和毒力基因携带状况研究[J].微生物与感染,2014,9(1):37-42.
[4] Velazquez-Roman J,Leon-Sicairos N,de Jesus Hemandez-Diaz L,et al.Pandemic Vibrio Parahaemolyticus O3:k6 on the American continent[J].Front Cell Infect Microbiol,2014,3(110):1-14.
[5] Nair GB,Ramamurthy T,Bhattacharya SK,et al.Global dissemination of VVibrio Parahaemolyticus serotype O3:K6 and its serovariants[J].Clin Microbiol Rev,2007,20(1):39-48.
[6] 韩海红,李宁,郭云昌.副溶血性弧菌分子标志基因研究情况[J].微生物学报,2015,55(1):12-21.
[7] 李涛,黎明,苗艳芳,等.成都市91株副溶血性弧菌血清型、耐药性及毒力基因检测[J].现代预防医学,2015,42(18):3376-3379,3419.
[8] 黄春梅,吴南卫,邓瑶.三亚地区副溶血性弧菌血清分型、毒力基因及药敏分析[J].现代预防医学,2016,43 (9):1665-1669.
[9] 吴靑,韩海红,余东敏.北京市水产品污染与感染病例中副溶血性弧菌血清型和毒力基因的比较研究[J].中国食品卫生杂志,2015,27(4):363-367.
[10] 林毅盈,潘力,吴清平,等.副溶血弧菌菌株229基因组测序及其致病因子的注释[J].现代食品科技,2018,34 (5):76-80.
[11] 王滨,吕均.一起副溶血弧菌污染小龙虾的病原学鉴定[J].中国卫生检验杂志,2011,21 (8):1969-1973.
[12] 王红,赵鹏,杜悦,等.副溶血性弧菌耐药传播及机制研究进展[J].中国卫生检验杂志,2018,28 (21):2685-2688.
[13] 袁媛,孙永,张钧,等.市售生食水产品污染副溶血性弧菌的病原学分析[J].安徽预防医学杂志,2013,19 (4):233-235,239.
[14] Bakeraustin C,Mcarthur JV,Tuckfield RC,et al.Antibiotic resistance in the shellfish pathogen Vibrio parahaemolyticus isolated from the coastal water and sediment of Georgia and South Carolina,USA[J].J Food Prot,2008,71 (12):2552-2558.
[15] Sudha S,Mridula C,Silvester R,et al.Prevalence and antibioticresistance of pathogenic Vibrios in shellfishes from Cochin market[J].Indian J Geo-Mar Sci,2014,43 (5):815-824.
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