Objective To understand the genotype characteristics of measles virus epidemic strains in Hefei City from 2017 to 2018,and to provide basis for measles prevention and control. Methods The throat swab specimens of measles cases from 2017 to 2018 were collected.RNA was extracted,and a 450-nucleotide region of the nucleoprotein gene N was amplified by RT-PCR.The sequence BLAST was performed on Genbank and virus subtype was determined by phylogenetic tree analysis. Results A total of 20 throat swab specimens were collected,of which 20 (15 in 2017,5 in 2018) specimens were successfully amplified N gene carboxyl terminal sequence.Sequence analysis and phylogenetic tree results showed that 17 strains (12 in 2017 and 5 in 2018) were H1a genotype,1 strain was H1 type,1 strain was D8 genotype,and 1 strain was highly homologous with vaccine strain Shanghai191 (U03663) (homology>99%). Conclusion H1a is still the dominant type of measles epidemic in Hefei City,and occasional imported cases have not caused the epidemic of new genotypes.It is necessary to strengthen the etiological identification of suspected measles cases in Hefei City and detect imported measles cases in time to avoid outbreaks and epidemics caused by new genotypes.
Key words
Measles virus /
Genotype /
Phylogenetic tree
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References
[1] 龙川凤,李忠洲.麻疹的流行病学特征及实验室检测研究进展[J].中国国境卫生检疫杂志,2019,42(5):376-380.
[2] 于霞丽,黄芳,付茵,等.2013年-2014年北京市流行麻疹病毒基因型特征分析[J].中国卫生检验杂志,2016,26(15):2132-2135.
[3] 李崇山,李智,王静,等.2018年上海市一起D8基因型麻疹病毒引起的输入性麻疹暴发报告[J].疾病监测,2019,34(7):672-675.
[4] de Swart,RL,Yüksel S,Osterhaus AD.Relative contributions of measles virus hemagglutinin-and fusion protein-specific serum antibodies to virus neutralization.J Virol[J].2005,79:11547–11551.
[5] World Health Organization,2012.World Health Organization.Measles Virus Nomenclature Update:2012.2012 Contract No.:87.Available at.https://www.who.int/wer/2012/wer8709.
[6] 李崇山,孙晓冬,唐伟,等.2012—2014 年上海市麻疹病毒学监测情况分析[J].微生物与感染,2016,11(1):12-17.
[7] 李智,崔晓娴,任佳,等.上海市2018年D8基因型麻疹病毒病例流行病学与病原学特征[J].中国疫苗和免疫,2019,25(6):630-634,663.
[8] Ciceri G,Canuti M,Bianchi S,et al.Genetic variability of the measles virus hemagglutinin gene in B3 genotype strains circulating in Northern Italy[J].Infect Genet Evol.2019,75:103943.
[9] Amendola A,Bubba L,Piralla A,et al.Surveillance and vaccination coverage of measles and rubella in Northern Italy[J].Hum Vaccin Immunother.2015,11 (1):206-213.
[10] Xu S,Zhang Y,Zhu Z,et al.2013.Genetic characterization of the hemagglutinin genes of wild-type measles virus circulating in china,1993–2009[J].PLoS ONE,2013,8(9),e73374.
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