Research progress of nucleic acid detection technology for SARS-CoV-2

CHAI Yu; TANG Ji-hai

doi:10.19837/j.cnki.ahyf.2021.03.014

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Anhui Journal of Preventive Medicine ›› 2021, Vol. 27 ›› Issue (3) : 223-228. DOI: 10.19837/j.cnki.ahyf.2021.03.014

Research progress of nucleic acid detection technology for SARS-CoV-2

CHAI Yu, TANG Ji-hai

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Abstract

Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) posed a serious threat to human health,and the resulting Corona Virus Disease 2019 (COVID-19) had swept the world.Therefore,rapid and accurate detection technology was a key means for early detection of the epidemic,which played an important role in effectively controlling the spread of the epidemic.Nucleic acid detection had become the most commonly used method for disease detection and screening due to its fast detection speed,good accuracy,and high sensitivity.This article will review the principles of Realtime PCR,loop-mediated isothermal amplification,digital PCR,and sequencing,as well as the application progress in the detection of COVID-19,hoping to provide a reference for SARS-CoV-2 detection and research.

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SARS-CoV-2 / COVID-19 / Nucleic acid detection

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CHAI Yu, TANG Ji-hai. Research progress of nucleic acid detection technology for SARS-CoV-2[J]. Anhui Journal of Preventive Medicine. 2021, 27(3): 223-228 https://doi.org/10.19837/j.cnki.ahyf.2021.03.014

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