Objective To understand the serotype,drug resistance spectrum and molecular typing characteristics of salmonella in Huangshan City. Methods Serotyping of 43 salmonella strains isolated and cultured in Huangshan City from 2019 to 2021 was performed,drug sensitivity test was performed by micro broth dilution method,molecular typing was performed by pulsed field gel electrophoresis (PFGE),and cluster analysis was performed by BioNumerics 7.6 software. Results There were 11 serotypes in 43 salmonella strains,among which salmonella enteritidis accounted for 44.19%(19/43) and salmonella typhimurium accounted for 30.23%(13/43).Salmonella had different degrees of drug resistance to 14 antibiotics,and the drug resistance rate of ampicillin was the highest (83.72%,36/43),followed by cefazolin (55.81%,24/43) and nalidixic acid (53.49%,23/43).Multidrug resistance results showed that the drug resistance rate of three or more antibiotics was 72.09% (31/43),and that of six or more antibiotics was 4.65% (2/43).The multidrug resistance rate of salmonella enteritidis was significantly higher than that of salmonella typhimurium,and the difference was statistically significant (χ²=13.095,P < 0.05).PFGE analysis showed that 43 salmonella strains produced 27 different PFGE bands,and the similarity between strains ranged from 43.70% to 100.00%,indicating that the genotypes of the same serotype were similar and homologous. Conclusion Salmonella serotypes and genotypes in Huangshan City are diverse,and the phenomenon of high drug resistance and multiple drug resistance is obvious.Therefore,the use and supervision of antibiotics should be strengthened.