Pathogenic diagnosis and genotype analysis of an acute gastroenteritis cluster epidemic

SONG Yongqing; JIN Jing; TANG Renshu; ZHU Yindi; ZHANG Wenyan

doi:10.19837/j.cnki.ahyf.2023.05.016

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Anhui Journal of Preventive Medicine ›› 2023, Vol. 29 ›› Issue (5) : 422-426. DOI: 10.19837/j.cnki.ahyf.2023.05.016

Disease Control

Pathogenic diagnosis and genotype analysis of an acute gastroenteritis cluster epidemic

SONG Yongqing, JIN Jing, TANG Renshu, ZHU Yindi, ZHANG Wenyan

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Abstract

Objective To understand the pathogen and genotype of an acute gastroenteritis cluster epidemic at a construction site in Hefei City. Methods Real time fluorescent PCR was used to screen the nucleic acid of norovirus,sapovirus and astrovirus in 16 anal swabs and 7 water samples.RT-PCR was used to amplify the gene of RdRp-VP1 region in norovirus nucleic acid positive samples.Gene sequencing was performed and viral genotype subtypes were determined by online comparison.MEGA software was used to build a gene evolution tree for gene comparison and evolution analysis. Results 14 anal swabs and 2 water samples were positive for norovirus nucleic acid,while both sapovirus and astrovirus nucleic acid were negative.9 gene sequences in the RdRp-VP1 region of norovirus were obtained,all of which were GⅡ.P17-GⅡ.17 type. Conclusion This is an acute gastroenteritis epidemic caused by drinking water contamination with norovirus type GⅡ.P17-GⅡ.17.

Key words

Norovirus / Gastroenteritis / Cluster epidemic / Pathogenic detection

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SONG Yongqing, JIN Jing, TANG Renshu, ZHU Yindi, ZHANG Wenyan. Pathogenic diagnosis and genotype analysis of an acute gastroenteritis cluster epidemic[J]. Anhui Journal of Preventive Medicine. 2023, 29(5): 422-426 https://doi.org/10.19837/j.cnki.ahyf.2023.05.016

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