Analysis of nucleic acid detection and isolation results of influenza virus in Huangshan City from 2020 to 2022

HU Wanqi; HUANG Lingling

doi:10.19837/j.cnki.ahyf.2023.06.007

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Anhui Journal of Preventive Medicine ›› 2023, Vol. 29 ›› Issue (6) : 472-476. DOI: 10.19837/j.cnki.ahyf.2023.06.007

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Analysis of nucleic acid detection and isolation results of influenza virus in Huangshan City from 2020 to 2022

HU Wanqi, HUANG Lingling

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Abstract

Objective To understand the situation of nucleic acid detection and isolation of influenza virus in Huangshan City from 2020 to 2022,so as to provide scientific basis for influenza prevention and improve the positive rate of influenza virus isolation. Methods Influenza-like cases were collected from 2020 to 2022 in Huangshan City.Real-time fluorescent quantitative RT-PCR was used to detect influenza virus nucleic acid.The positive nucleic acid samples of influenza virus were isolated by Madin-Darby canine kidney cells (MDCK cells).The hemagglutination (HA) and hemagglutination inhibiion (HI) tests were used for identification and typing. Results A total of 3 417 throat swab samples of influenza-like cases were detected in Huangshan City from 2020 to 2022,and 402 positive nucleic acid specimens were detected,with a detection rate of 11.76%.The positive detection rates of influenza virus nucleic acid from 2020 to 2022 were 7.10%,4.59% and 21.52%,respectively,with statistical significance (χ²=193.563,P<0.001).B-Victoria and H3 were dominant types.The positive rate of influenza virus nucleic acid was the highest in the age group of 6-15 years,which was 17.05% (75/440).Among 297 influenza virus nucleic acid positive samples,188 strains of influenza virus were successfully isolated,and the positive rate of virus isolation was 63.30%.The isolation positive rates of B-Victoria and H3 influenza viruses in the first and second generations were 27.95% and 63.30%,respectively,with significant difference (χ²=74.816,P< 0.001). Conclusion B-Victoria and H3 are the dominant types of influenza in Huangshan City from 2020 to 2022.The highest positive rate of influenza virus nucleic acid is in the age group of 6-15 years.The positive rate of influenza virus isolation can be greatly improved by passing two generations.

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Influenza virus / Nucleic acid testing / Virus isolation

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HU Wanqi, HUANG Lingling. Analysis of nucleic acid detection and isolation results of influenza virus in Huangshan City from 2020 to 2022[J]. Anhui Journal of Preventive Medicine. 2023, 29(6): 472-476 https://doi.org/10.19837/j.cnki.ahyf.2023.06.007

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