Objective To investigate the first-time HIV antibody testers among men who have sex with men (MSM) population in a certain district of Beijing City,so as to understand their knowledge,attitude,behavior status,and HIV infection status. Methods The "snowball" method was used to find respondents to the survey,providing them with rapid HIV antibody testing services,and conducting face-to-face questionnaire surveys.SPSS 18.0 was used for statistical analysis. Results A total of 395 subjects were included in this survey,with an average age of (32.0±6.8) years old.The proportion of unmarried was 63.8% (252/395),the proportion of bachelor's degree or above was 54.4% (215/395),and the proportion of homosexual orientation was 69.9% (276/395).91.4% (361/395) of those who sought gay partners online through internet platforms,and 74.4% of them knew about HIV/AIDS.The mean age of first sexual intercourse was (26.0±5.2) years,and the minimum age was 13 years.In the last six months,23.8% (94/395) had unprotected homosexual sex,96.2% (380/395) had sex with one to three people,38.2% (151/395) had intercourse with female,62.3% (94/395) used condoms every time they had sex with the female.The HIV antibody positive rate was 8.1% (32/395),and the HIV infection rate among those who engage in unprotected sexual activity was 23.4% (22/94) in the past six months. Conclusion The positive rate of HIV antibody of first-time HIV antibody testers among MSM population in a certain district of Beijing City is high,the awareness rate of AIDS knowledge is low,and there are high-risk behaviors of HIV infection.