Objective To analyze the HIV genotype,drug resistance characteristics before treatment and HIV molecular transmission network of newly reported HIV infections from 2021 to 2022 in Bengbu City,Anhui Province,in order to provide a basis for the formulating of local intervention measures. Methods 90 samples of newly reported HIV infections in Bengbu City from 2021 to 2022 were collected,pol genes were amplified and sequenced,the homology analysis of each sequence was analyzed with the help of Blast tool HIV sequence database,and MEGA 7.0 was used to construct the NJ system evolutionary tree to determine HIV genotypes of all HIV infections.The resulting sequences were uploaded to the HIV resistance database of Stanford University to analyze genotype resistance.The transmission network was visualized by Cytoscape to analyze the transmission characteristics of drug resistance and the network association of newly reported HIV infections in Bengbu City. Results The main genotypes of 85 newly reported HIV infections in Bengbu City were CRF01_AE (58.82%) and CRF07_BC(23.53%),the drug resistance rate was 10.59% (9/85).It was mainly resistant to non-nucleoside reverse transcriptase inhibitors(5/85,5.88%).Analysis of the molecular propagation networks at the 0.5% gene distance threshold,the CRF01_AE access rate was 44%,and there was a large resistance spread cluster in which the resistance site was L33F.The CRF07_BC access rate was 10%.The main HIV infected individuals in the molecular transmission network were males aged 50 and above in Wuhe County,with a relatively low level of education. Conclusion The drug resistance rate of newly reported HIV infections in Bengbu City is high,forming a drug-resistant transmission cluster and posing a high risk of transmission.Effective measures should be taken to reduce the transmission of HIV.
Key words
HIV /
Drug resistance /
Molecular transmission network
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