Genomic characteristics of Listeria monocytogenes isolated in Huainan City from 2021 to 2022

ZHANG Jie; SUN Mengyang; WANG Yuqiu; WANG Xiaobo; ZENG Fanrong; YU Shoujie; LIU Jiang

doi:10.19837/j.cnki.ahyf.2024.05.006

PDF(841 KB)

Anhui Journal of Preventive Medicine ›› 2024, Vol. 30 ›› Issue (5) : 370-374. DOI: 10.19837/j.cnki.ahyf.2024.05.006

Original Articles

Genomic characteristics of Listeria monocytogenes isolated in Huainan City from 2021 to 2022

ZHANG Jie, SUN Mengyang, WANG Yuqiu, WANG Xiaobo, ZENG Fanrong, YU Shoujie, LIU Jiang

Author information +

History +

Abstract

Objective To understand the molecular types,virulence genes and drug resistance genes of Listeria monocytogenes,and to provide scientific basis for prevention and control of it. Methods 10 strains of Listeria monocytogenes isolated in Huainan City from 2021 to 2022 were subjected whole genome sequencing.The molecular types of strains were analyzed online through BIGSdb database,virulence genes and drug resistance genes were analyzed by bioinformatics analysis software. Results Among 10 Listeria monocytogenes strains,9 were isolated from food and 1 was isolated from a clinical patient.They were divided into 6 sequences types (ST),ST8 (50%) was dominant,and the clinical isolates were ST3,which was different from the food isolates.All strains carried virulence islands LIPI-1 and LIPI-2,and only ST3 strains carried LIPI-3 with the highest number of detected virulence genes.Four strains (ST3,ST1032,ST87,ST91) carried vip virulence genes,all strains carried FosX,mprF,lin,and norB resistance genes. Conclusion The strains of Listeria monocytogenes in Huainan City carried various molecular types,virulence genes and drug resistance genes.Special attention should be paid to strains carrying vip genes.The clinical isolates were highly virulent and different from food isolates,so the monitoring of food samples should be strengthened.

Key words

Listeria monocytogenes / Whole genome sequencing / Virulence gene / Drug resistance gene

Cite this article

EndNote

Ris (Procite)

Bibtex

Download Citations

ZHANG Jie, SUN Mengyang, WANG Yuqiu, WANG Xiaobo, ZENG Fanrong, YU Shoujie, LIU Jiang. Genomic characteristics of Listeria monocytogenes isolated in Huainan City from 2021 to 2022[J]. Anhui Journal of Preventive Medicine. 2024, 30(5): 370-374 https://doi.org/10.19837/j.cnki.ahyf.2024.05.006

References

[1] 张晶,白志军,和鹏,等.广州市58株单增李斯特菌菌株特征分析[J].现代预防医学,2019,46(5):879-883.
[2] Koopmans MM,Brouwer MC,Vázquez-Boland JA,et al.Human listeriosis[J].Clin Microbiol Rev,2023,36(1):e0006019.
[3] 霍哲,张晓嫒,徐俊,等.2015—2019年北京市西城区人源性单核细胞增生李斯特菌的分子特征分析[J].疾病监测,2021,36(3):270-275.
[4] Thomas J,Govender N,McCarthy KM,et al.Outbreak of listeriosis in South Africa associated with processed meat[J].N Engl J Med,2020,382(7):632-643.
[5] Fernández-Martínez NF,Ruiz-Montero R,Briones E,et al.Listeriosis outbreak caused by contaminated stuffed pork,Andalusia,Spain,July to October 2019[J].Eur Commun Dis Bull,2022,27(43):2200279.
[6] Palacios A,Otto M,Flaherty E,et al.Multistate outbreak of Listeria monocytogenes infections linked to fresh,soft hispanic-style cheese - United States,2021[J].MMWR Morb Mortal Wkly Rep,2022,71(21):709-712.
[7] 辛永萍,单颖,夏叶.一株单核细胞增生李斯特菌分离株的分子分型鉴定及其生物学特性[J].畜牧兽医学报,2020,51(5):1101-1109.
[8] 刘世芸,王圣庆,刘璞.池州市2014—2015年食品中食源性致病菌调查结果分析[J].安徽预防医学杂志,2016,22(2):107-109.
[9] 吴莉丹,冉雪琴,牛熙,等.猪源致病性大肠杆菌基因组比较与毒力因子分析[J].生物技术通报,2023,39(12):287-299.
[10] Lakicevic B,Jankovic V,Pietzka A,et al.Wholegenome sequencing as the gold standard approach for control of Listeria monocytogenes in the food chain[J].J Food Prot,2023,86(1):100003.
[11] 孙婷婷,孙岐峰,王伟杰,等.2016—2018年辽宁省食品中单核细胞增生李斯特菌污染情况及毒力基因的检测[J].中国微生态学杂志,2020,32(2):156-160.
[12] 李秀娟,赵冬,潘琢,等.29种毒力基因在91株食源性单核细胞增生李斯特氏菌中的分布[J].中国人兽共患病学报,2017,33(11):972-978.
[13] Tavares RM,Silva DALD,Camargo AC,et al.Interference of the acid stress on the expression of llsX by Listeria monocytogenes pathogenic island 3 (LIPI-3) variants[J].Food Res Int,2020,132:109063.
[14] Zhang H,Chen W,Wang J,et al.10-year molecular surveillance of Listeria monocytogenes using whole-genome sequencing in Shanghai,China,2009-2019[J].Front Microbiol,2020,11:551020.
[15] 郝歌,钱映,李蓉,等.单核细胞增生李斯特菌毒力基因及其致病机制的研究进展[J].中国食品卫生杂志,2023,35(3):481-486.
[16] 苏乐斌,李柏生,谭海芳,等.基于全基因组测序的单核细胞增生李斯特菌食品分离株分子特征分析[J].中国食品卫生杂志,2019,31(6):522-527.
[17] 李森,牟俊洁,刘武康.单增李斯特菌vip基因缺失菌株的构建与筛选[J].微生物学杂志,2017,37(4):16-22.
[18] 王小龙,汤全英,邹文燕,等.苏州市食源性单核细胞增生李斯特菌的全基因组测序分析[J].食品安全质量检测学报,2023,14(8):255-261.
[19] Mota MI,Vázquez S,Cornejo C,et al.Does shiga toxin-producing Escherichia coli and Listeria monocytogenes contribute significantly to the burden of antimicrobial resistance in Uruguay?[J].Front Vet Sci,2020,7:583930.
[20] Maury MM,Tsai YH,Charlier C,et al.Erratum:uncovering Listeria monocytogenes hypervirulence by harnessing its biodiversity[J].Nat Genet,2017,49(6):970.
[21] Doumith M,Buchrieser C,Glaser P,et al.Differentiation of the major Listeria monocytogenes serovars by multiplex PCR[J].J Clin Microbiol,2004,42(8):3819-3822.
[22] 康立超,钱晶,杜冬冬,等.新疆食源性单增李斯特菌MLST分型及耐药性分析[J].食品安全质量检测学报,2021,12(15):6255-6261.
[23] 成梦雅,王文凯,史贤明,等.上海市食源性单核细胞增生李斯特菌MLST分析及毒力基因分布[J].中国食品学报,2019,19(2):223-229.
[24] 林慧琳,叶玲清,叶海梅,等.2011—2021年福建省即食食品中单增李斯特菌的监测及菌株特征分析[J].中国人兽共患病学报,2023,39(10):941-950.
[25] 宋泽萱,纪顺师,王艳,等.中国单增李斯特菌的基因组特征研究[J].中国人兽共患病学报,2021,37(5):379-386.