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Analysis of laboratory detection results for a foodborne outbreak caused by Vibrio parahaemolyticus
ZHAO Yu, LIU Yuwei, LI Shoufei, WANG Miao, WANG Luotong, LI Ying, ZHANG Shuang
Anhui Journal of Preventive Medicine ›› 2025, Vol. 31 ›› Issue (5) : 411-415.
PDF(761 KB)
PDF(761 KB)
Analysis of laboratory detection results for a foodborne outbreak caused by Vibrio parahaemolyticus
Objective To investigate the etiological characteristics of a diarrhea outbreak caused by Vibrio parahaemolyticus (VP) infection,and to provide a basis for further prevention and control.Methods Epidemiological information related to the outbreak was collected,and samples from cases,chefs,food,and environmental swabs were collected.Real-time PCR was used to screen for common diarrhea-causing pathogens.VP was isolated and identified from the original samples and enrichment broth,and the obtained VP strains were serotyped and tested for characteristic and virulence genes.The drug resistance of the strains was determined by broth micro-dilution method,and the genetic evolution of the strains whole genome sequences was analyzed.Results After the enrichment solution was cultured,one strain of VP (toxR+/tdh+) was isolated from each anal swab of 4 cases,one strain of VP (toxR+/tdh+) was isolated from each anal swab of 3 chefs,and seven strains of VP (toxR+/tdh-) were isolated from 1 environmental sample.The serotypes of VP strains isolated from humans were all O10:K4.The 7 strains of VP isolated from rectal swabs were all resistant to ampicillin and polymyxin E,showed clonal genetic characteristics.The 7 strains of VP isolated from the environment had different resistance phenotypes,showed genetic diversity.Conclusion The event was caused by monoclonal serotype VP infection,and whole-genome sequencing technology has a good application prospect in the etiological analysis of diarrhea outbreaks.
Vibrio parahaemolyticus / Foodborne diseases / Whole genome sequencing
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