Objective To Understand the pathogens of the first case of human H7N9 infections. Methods The respiratory specimens were collected from a case with pneumonia of unkno-wn cause reported in Wuhu city, Viral RNA was extracted from the specimens and detected on specif-ic genes by real-time RT-PCR, The specimens were submitted to CNIC for gene sequencing. Results FluA virus and specific H7N9 subtype from the resp,ratory tract estract were positive, The nucleotide acid sequences of HA and NA gene were highly similar with ot-her known avian isolate strain sequences(HA: 99.4%~99.8%;NA: 99.2%~100.0%), The highest similarit-y of nucleotide sequence of HAgene was KM054796-A/chicken/Jilin/2014,The highest similarity of NA gene was KP414837-A/chicken/Jiangxi/2014. there was the closest evolutionary relationships with KP414837-A/chicken/Jiangxi/2014. Conclusion The viral HA and NA sequences of the first case of human infection with H7N9 avian influenza in wuhu city were highly homologous to the known H7N9 avian influenza virus.
Key words
Avian influenza /
H7N9 /
Real-time RT-PCR
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References
[1] 张宝,黄克勇,郭劲松,等.H7N9病毒的来源和重组模式[J].南方医科大学学报,2013,33(7):1017-1021.
[2] WHO.Real-time RT PCR(rRT-PCR) Protocol for the Detection of Avian Influenza A(H7N9) Virus [EB/OL].(2013-04-15)[2013-05-05].http://www.who.int/influenza/gisrs_laboratory/cnic_realtime_rt_pcr_protocol_a_h7n9.pdf.
[3] 聂轶飞,王海峰,康锴,等.人禽流感病原学与流行病学相关研究[J].河南预防医学杂志,2011,22(6):401-404.
[4] 中华人民共和国国家卫生和计划生育委员会.人感染H7N9禽流感诊疗方案(2014年版)[Z].传染病信息,2014,27(01):1-4.
[5] Gao R, Cao B, Hu Y,et al.Human infection with a novel avian-origin influenza A (H7N9) virus[J].The New England journal of medicine.2013;368(20):1888-1897.
[6] Sleeman K, Guo Z, Barnes J et al.R292K substitution and drug susceptibility of influenza A(H7N9) viruses[J].Emerging infectious diseases.2013,19(9):1521-1524.
[7] Chen Y, Liang W, Yang S,et al.Human infections with the emerging avian influenza A H7N9 virus from wet market poultry: clinical analysis and characterisation of viral genome[J].Lancet (London, England),2013,381(9881):1916-1925.
[8] 安洲,程周祥,钱帮群,等.芜湖市首例人感染H7N9流感病例调查报告[J].安徽预防医学杂志,2015,21(04):249-251.
[9] 张兰荣,赵凤玲,罗宇馨,等.应用3种流感病毒检测方法检测流感样病例病原结果及分析[J].中国卫生检验杂志,2010,20(05):1079-1080,1085.
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