Objective To analyze the results of laboratory test of suspected measles cases in Bengbu city in 2015, and understand the prevalence of measles. Methods The IgM antibody of measles in serum was detected by ELISA, and the measles virus nucleic acid was detected by fluorescence quantitative PCR method. Results There were 261 IgM positive samples detected in total 784 serum samples, and the positive rate was 33.29%. There were 67 measles virus nucleic acid positive samples detected in total 128 throat swab samples, and the positive rate was 52.34%. Conclusion Complementary application of serological detection and nucleic acid detection could improve the detection rate in the diagnosis of measles.
Key words
Measles virus /
ELISA /
Fluorescence quantitative PCR /
Measles antibody /
Measles virus nucleic acid
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References
[1] 中华人民共和国卫生部.2006～2012年全国消除麻疹行动计划[Z]. 2006.
[2] 欧阳霖,尤佳恺,陈国强,等. 疑似麻疹病例不同采血时间的IgM抗体检测分析[J].中国实用医药,2011,6(30):81-82.
[3] 刘建锋,孟蕾,李慧,等.甘肃省麻疹野病毒流行株分离鉴定研究[J].卫生职业教育,2009,27(7):111-112.
[4] 高丹,张国斌,贾妮娜.实时荧光RT-PCR法快速检测麻疹病毒核酸[J].中国卫生检验杂志,2015,25(11):1815-1819.
[5] 李艳芬,黎景全,袁达康,等.2013~2014年东莞市麻疹监测结果分析[J].中国卫生检验杂志,2015,25(21):3731-3736.
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