Analysis of molecular characteristics of norovirus GII.4/Sydney 2012 variant in Anhui province

SHI Yong-lin; ZHANG Wen-yan; JIN Jing; ZHOU Wei; TANG Ren-shu; WU Jin-ju; JIN Miao; HU Wan-fu

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Anhui Journal of Preventive Medicine ›› 2016, Vol. 22 ›› Issue (6) : 363-366.

Analysis of molecular characteristics of norovirus GII.4/Sydney 2012 variant in Anhui province

SHI Yong-lin¹, ZHANG Wen-yan², JIN Jing², ZHOU Wei², TANG Ren-shu², WU Jin-ju², JIN Miao³, HU Wan-fu¹

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Abstract

Objective To understand the genotype and molecular characteristics of the pathogens associated with the epidemic outbreak of acute gastroenteritis in a university of Anhui province. Methods A total of 6 fecal(anal swab)samples from acute gastroenteritis outbreak were collected. Norovirus (NoV) nucleic acid was detected by using Real-time PCR, and the partial ORF2 of the all positive specimens were amplified by RT-PCR and sequenced. Phylogenetic tree was constructed by using Mega6.0 based on partial ORF2 sequences of norovirus to perform phylogenetic analysis. Results Of the 4 specimens, 4(66.67%)were positive for NoV, and two positive samples were sequenced successfully. Phylogenetic analysis showed that 2 strains belonged to genotype GII.4/Sydney 2012 norovirus. The nucleotide homology between the 2 partial ORF2 sequences was 100%. The regions had 99.6% and 100% nucleotide amino identity compared with Sydney 2012 strain (JX459908) in Australia and Shanghai/HK strains in 2014-2105, respectively. Conclusion The epidemic outbreak of acute gastroenteritis in Anhui province was caused by genotype GII.4/Sydney 2012.

Key words

Norovirus / G II.4 genotype / Sydney 2012 / Molecular characteristics

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SHI Yong-lin, ZHANG Wen-yan, JIN Jing, ZHOU Wei, TANG Ren-shu, WU Jin-ju, JIN Miao, HU Wan-fu. Analysis of molecular characteristics of norovirus GII.4/Sydney 2012 variant in Anhui province[J]. Anhui Journal of Preventive Medicine. 2016, 22(6): 363-366

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