Objective To explore the association of TCF7L2 gene polymorphisms and gene-environmental interaction with type 2 diabetes mellitus (T2DM.). Methods A 1:1matched case control study was conducted, and 298 cases of T2DM and 298 controls were selected from community population in 5 national disease surveillance points of Fujian province. The questionnaire survey, physical examination and laboratory examination were carried out. The genotypes of 10 SNP loci of TCF7L2 gene (rs11196205, rs12255372, rs4506565, rs7901695, rs7903146, rs7895340, rs11196218, rs12775879, rs290481, rs6585205) were detected by MALDI-TOF-MS. Genotype distribution frequency and haplotype were analyzed by SHEsis online software. Generalized multifactor dimensionality reduction (GMDR) method was used to evaluate gene-environmental interactions. Unconditional logistic regression model was used to analyze the risk of gene-environmental interaction. Andersson Excel table was used to evaluate additive interaction indicators, such as the relative excess risk due to interaction (RERI), the attributable percent (AP), and the interaction index (S). Results There were no significant differences of 10 SNPs distribution of TCF7L2 between the case group and control group.Central obesity is the main effect factor of T2DM. There were significant differences of HOMA-β among the three genotypes of rs290481 in the case group(P<0.05). Conclusion Central obesity played a major role in T2DM in this study. The polymorphism of rs290481 of TCF7L2 gene may affect insulin secretion function of pancreatic islet β cell.
Key words
Diabetes mellitus /
Type 2 /
TCF7L2 /
Gene polymorphism /
Environment /
Interaction
{{custom_sec.title}}
{{custom_sec.title}}
{{custom_sec.content}}
References
[1] Ding D,Chong S,Jalaludin B,et al.Riskfactorsofincidenttype2-diabetesmellitusovera3-yearfollowup:ResultsfromalargeAustraliansample[J].DiabetesResClinPract,2015,108(2):306-315.
[2] Grant SF,Thorleifsson G,Reynisdottir I,et al.Variantoftranscriptionfactor7-like2(TCF7L2)geneconfersriskoftype2diabetes[J].NatGenet,2006,38(3):320-323.
[3] 赵珂,李新华,丁文宇.TCF7L2基因单核苷酸多态性与2型糖尿病关系研究进展[J].山东医药,2016,56(14):99-101.
[4] 罗维,余杨文,郭志来,等.TCF7L2基因rs12255372和rs7903146多态性与2型糖尿病发生研究进展[J].临床医学研究与实践,2016,1(1):120-121.
[5] Miyake K,Horikawa Y,Hara K,et al.AssociationofTCF7L2 polymorphisms with susceptibility to type 2 diabetes in 4087 Japanese subjects[J].J Hum Genet,2008,53(2):174-178.
[6] Ren Q,Han XY,Wang F,et al.Exon sequencing and association analysis of polymorphisms in TCFTL2 with type 2 diabetes in a Chinese population[J].Diabetologia,2008,51(7):1146-1152.
[7] Ng MC, Tam CH, Lam VK, et al.Replicafion and identification of novel variants at TCF712 associated with type 2 diabetes in Hong Kong Chinese[J]. J Clin Endocrinol Metab, 2007, 92(9) : 3733-3737.
[8] 黄争春,邱伟,董明华,等.膳食、生活方式等环境因素对T2DM的影响[J].赣南医学院学报,2015,35(2):208-211.
[9] 牛洁,刘铜华,杨丽霞,等.环境因素在2型糖尿病发生中的作用[J].中国慢性病预防与控制,2008,16(4):440-442.
[10] 张留伟,李劲,段芳芳,等.TCF7L2和KCNQ1基因多态性与2型糖尿病的关联[J].环境与健康杂志,2014,31(2):156-159.
[11] 张京,董忠,李刚,等.北京市居民2型糖尿病现况调查和危险因素研究[J].中华流行病学杂志,2011,32(4):357-360.
[12] 刘立亚,陈立章,戴进雄,等.湘西地区侗族2型糖尿病危险因素的病例对照研究[J].中南大学学报(医学版),2013,38(10):1057-1064.
[13] 陶然,徐燕,苏健,等.江苏省成年人肥胖测量指标与糖尿病患病风险的关系[J].中华糖尿病杂志,2014,6(10):742-747.
[14] Schöfer SA,Tschritter O,Machicao F,et al. Impaired glucagon-like peptide-1-induced insulin secretion in carriers of transcription factor 7-like 2 (TCF7L2)gene polymorphisms[J].Diabetologia,2007,50 (12):2443-2450.
[15] Zhou Y,Zhang E,Berggreen C,et al. Survival of pancreatic beta cells is partly controlled by a TCF7L2-p53-p53INP1-dependent pathway[J].Hum Mol Genet,2012,21(1) : 196-207.
PDF(811 KB)
