Objective To establish a method for simultaneous determination of the chloramphenicol (CAP)and metronidazole (MNZ)in honey by a solid phase extraction coupled to ultra high performance liquid chromatography-tandem mass spectrometry ( UPLC-MS/MS ). Methods CAP and MNZ in honey samples extracted by optimization of solid phase extraction method, then separated in UPLC, the 25 honey samples of Hefei local were simultaneous determined using mass spectrometry ESI- and ESI + mode and quantified by deuterated isotope internal standard method, also tested precision and recovery by adding low, medium and high concentration of standard solution. Results Good linear response were obtained from CAP and MNZ with the correlation coefficients(r) exceeding 0.9992. When sampling amount was 5.0 g, the limits of detection(LOD)or CAP and MNZ in honey were 0.001 μg/kg . The average recoveries at three spiked levels and the relative standard deviation( RSD) ranged from 93.6% to 105.0% and within 2.2% ~7.6%, respectively. Conclusion The method has advantages such as sensitive, accurate, reliable, and suitable for the analysis of quality and control method for trace CAP and MNZ in honey.
Key words
UPLC-MS/MS /
Soild phase extraction /
Chloramphenicol /
Metronidazole /
Honey
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