Objective To study the antibacterial efficacy and safety of a compound plant essential oil. Methods The bacteriostasis test and stability test of Staphylococcus aureus, Escherichia coli and Candida albicans with compound plant essential oil were used to observe the bacteriostasis performance. The compound plant essential oil was coated on the back skin of the healthy guinea pig, 14d was applied continuously, and the negative control was adopted, that is, no measures was taken, and the skin of guinea pig was observed to be irritating. Inflammatory reaction revealed the bacteriostatic property and safety of this essential oil. Result The diameter of the inhibition circle for Escherichia coli, Staphylococcus aureus, Candida albicans were more than 7mm, and effect was stable. When the plant essential oil was placed under 37 centigrade and 75% relative humidity for 3 months, the bacteriostasis performance was basically the same as before the storage. The skin irritation index was 0 and the intensity had no irritation. Conclusion The compound plant essential oils has good antibacterial effect to bacteria and fungal, stable property and safety without irritation to the skin.