Objective To investigate the water iodine content of drinking water and high iodine village's distribution in Bengbu, and give the guidance for scientific iodine supplement. Methods The investigation of first level of iodine content in drinking water was carried out in three counties and four districts of Bengbu. Choosing an administrative village to survey iodine content from five directions, the second level sampling survey was carried out in the villages of a village with a median of water vodime >10 μg/L. Taking the administrative village as the basic unit, to the concentrate water supply villages, only one part of the end of the pipe network; For the dispersed water supply administrative villages, each village shall adopt two water samples according to the five directions; When the waters iodine are less than 10 μg/L, collected all. The iodine content of drinking water in each village was determined by using water iodine detection method suitable for iodine deficiency and high iodine area, and the standard was referred to the demarcation of water source high iodine area and high iodine disease area,determine the high iodine water administrative villages. Results In the first level, 607 samples of water samples were detected, the median of water iodine was 11.3 μg/L, and the iodine content in 41 towns was >10 μg/L. In the second level, 3084 samples of water samples were detected, and the median of water iodine was 22.8 μg/L.The number of high water iodine administrative villages was 132, accounting for 17.9%. The water iodine content in the distributed water supply area was negatively correlated with the depth of the wells.r =-0.049,P=0.013 (P<0.05). The water iodine content in the centralized water supply area was positively correlated with the depth of the wells.r= 0.542,P= 0.000 (P< 0.05). Obviously the water iodine content was related to the depth of the well. Conclusion Bengbu city had both iodine deficiency areas and high iodine ones.Some administrative villages were water iodine contents of 10~100 μg/L, whether they needs to be filled with iodine should further study.