Etiological analysis of an outbreak of acute respiratory infection in a primary school in Hefei

CHEN Jiong; JIN Jing; ZHOU Huxiao; ZHAO Kefu; TANG Renshu; ZHANG Wenyan

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Anhui Journal of Preventive Medicine ›› 2018, Vol. 24 ›› Issue (4) : 247-249.

Etiological analysis of an outbreak of acute respiratory infection in a primary school in Hefei

CHEN Jiong¹, JIN Jing¹, ZHOU Huxiao², ZHAO Kefu¹, TANG Renshu¹, ZHANG Wenyan¹

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Abstract

Objective To analyze the epidemiological characteristics and identify the pathogen of an acute respiratory tract infection in a school in Hefei. Methods A total of 10 nasal and throat swab samples from the acute respiratory tract infection were collected, influenza virus and respiratory adenovirus(ADV) nucleic acid were detected by real-time PCR. Respiratory adenovirus(ADV) positive samples were amplified the Hexon gene of ADV further via polymerase chain reaction(PCR). The PCR product was sequenced and the sequences were compared in the Gen Bank to confirm their subtype and analyze phylogenetree evolution. Results Among the 10 nasal and throat swabs samples, the nucleic acid of influenza virus was negative, and 7(7/10) were ADV positive. The adenovirus 3 was confirmed by gene sequencing, sequence alignment and gene evolution tree analysis. Conclusion Based on laboratory test results, epidemiological investigation and clinical symptoms, human adenovirus type 3 was identified as the pathogen of the acute respiratory infection.

Key words

Adenovirus / Acute respiratory infection / Outbreak / Six ortho gene / Molecular biology

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CHEN Jiong, JIN Jing, ZHOU Huxiao, ZHAO Kefu, TANG Renshu, ZHANG Wenyan. Etiological analysis of an outbreak of acute respiratory infection in a primary school in Hefei[J]. Anhui Journal of Preventive Medicine. 2018, 24(4): 247-249

References

[1] Binder AM,Biggs HM,Haynes AK,et al.Human adenovirus surveillance - United States, 2003-2016[J].MMWR Morb Mortal Wkly Rep,2017, 66(39): 1039-1042.
[2] 陈伟,王盛书,张文义,等.我国呼吸道腺病毒疾病流行病学现况分析[J].军事医学,2017,10:814-821.
[3] 吴巨龙,刘倜,张圣洋,等.山东省某中学一起腺病毒引起的呼吸道感染暴发的调查[J].中国卫生检验杂志,2017,27(1):129-130,134.
[4] 曹孟婵,刘敏鸿,汪金生,等.安庆市呼吸道感染疫情人腺病毒的病原分析[J].安徽预防医学杂志,2015,21(1):1-3,7.
[5] 汪东篱,姚炜,郑庆鸣,等.一起腺病毒引起的急性呼吸道感染暴发疫情的调查研究[J].病毒学报,2017,33(6):920-924.
[6] 刘滕颖子,吕星,黄达娜,等.深圳市儿童呼吸道腺病毒4型暴发流行的病原学研究[J].中华实验和临床病毒学杂志,2015, 29 (2):115-117.
[7] Kajon AE,Lamson DM, Bair CR,et al.Adenovirus type 4 respiratory infections among civilian adults,northeastern United States, 2011-20151[J].Emerg Infect Dis,2018,24(2):201-209.
[8] Zheng X, Rong X, Feng Y, et al.Seroprevalence of neutralizing antibodies against adenovirus type 14 and 55 in healthy adults in Southern China[J].Emerg Microbes Infect,2017,6(6):e43.
[9] Yoo H, Gu SH, Jung J,et al.Febrile respiratory illness associated with human adenovirus type 55 in south Korea military, 2014-2016[J].Emerg Infect Dis,2017,23(6):1016-1020.
[10] Alkhalaf MA, Guiver M, Cooper RJ.Genome stability of adenovirus types 3 and 7 during a simultaneous outbreak in Greater Manchester, UK[J].J Med Virol,2015,87(1):117-124.
[11] 赵寒,李勤,龙江,等.一起腺病毒引起的急性呼吸道感染爆发调查分析[J].重庆医学,2015,44(4):506-508.
[12] 蒋露芳,居丽雯,姜仁杰,等.腺病毒3型急性呼吸道感染暴发流行的病原学调查[J].中华传染病杂志,2006,24(1):3-6.
[13] 陈应坚, 李静媚, 周健明,等.1起由腺病毒4型引起的呼吸道感染暴发疫情的流行病学分析[J].华南预防医学,2014,40(5):472-474.
[14] 刘仰青, 吴景文, 李辉,等.一起由腺病毒7型引起的呼吸道传染病聚集性疫情的流行病学调查[J].现代预防医学,2014,41(19):3481-3483.
[15] 黎燕, 李江波, 朱丹,等. 一起腺病毒3型引起暴发疫情的调查[J]. 浙江预防医学,2014,26(5):503-504.
[16] 王慎骄, 龚利强, 陈立凌,等.一起聚集性腺病毒暴发疫情的调查及病原学分析[J].江苏预防医学,2017,28(4):407-410.
[17] 解艳涛,杨艳娜,李洪军,等.一起腺病毒3型引起的小学呼吸道感染暴发疫情的流行病学调查[J].疾病监测,2016,31(11):972-974.
[18] 廖刚, 彭文鸿,谢扬新,等.聚集性人B组7型腺病毒性肺炎218例临床特征分析[J].中华肺部疾病杂志(电子版),2015,8(6):54-57.