Objective To explore the effect and feasibility of PBL combined with Seminar teaching method in clinical practice teaching of Hematology. Methods The undergraduates of clinical specialty in hematology practice were randomly divided into two groups.The experimental group consisted of 50 students, who accepted PBL combined with Seminar teaching method.The control group consisted of 52 students, who accepted traditional clinical teaching method.The questionnaire and personal interviews were used to evaluate the teaching effect of the course, and entrance examination was used to evaluate the scores of the two groups. Results It could be see from the evaluation of the teaching effect of the experimental group that most of the students (64.0%) could accept the teaching mode, most of the students said that the teaching mode could improve their interest in learning (60.0%) and their abilities in many aspects.52% of the students were willing to continue the program.The scores of the students in the experimental group were higher than those in the control group in both theoretical and practical skills tests, and the difference between the two groups was statistically significant (P<0.001). Conclusion The "PBL-Seminar"teaching mode is a novel and effective clinical teaching method, which can be appropriately adopted in the clinical teaching of hematology to improve students' initiative and cognitive ability in learning.