Objective To understand the characteristics of confirmed and uncertain specimens in AIDS confirmation test, and to analyze the band distribution of confirmed and uncertain specimens. Methods HIV antibody first screening positive samples were collected from HIV screening laboratories of various testing institutions in municipal districts of Bengbu City from 2008 to 2017. According to the requirements of National AIDS Testing Technical Specification, the confirmation test of HIV antibody was carried out by western blotting method (WB method). Results During the 10-year period, 46 145 samples were screened and tested for AIDS. Among them, 1 041 were confirmed positive for AIDS antibodies, with a positive rate of 2.26%. The number of uncertain antibodies was 122, with the exception of 2015, the uncertain figures increased year by year. There was no difference in specificity and sensitivity between domestic reagents. Conclusion Western blotting method was widely used in the confirmation of AIDS cases and plays an important role in the prevention and treatment of AIDS.